Microfluidic baker's transformation device for three-dimensional rapid mixing.
Microfluidic baker's transformation device for three-dimensional rapid mixing.
复制标题
DOI：
10.1039/c1lc20342h
复制
发表时间：
2011-09
期刊：
影响因子：
6.1
通讯作者：
T. Yasui;Yusuke Omoto;K. Osato;N. Kaji;N. Suzuki;T. Naito;Masaki Watanabe;Y. Okamoto;M. Tokeshi
中科院分区：
文献类型：
--
作者：
T. Yasui;Yusuke Omoto;K. Osato;N. Kaji;N. Suzuki;T. Naito;Masaki Watanabe;Y. Okamoto;M. Tokeshi
我们研制了一种基于面包师变换的新型被动型微混合器，实现了扩散常数较小的蛋白质溶液的快速混合。面包师变换是一种理想的混合方法，但由于需要制作复杂的三维(3D)结构来实现微流控面包师变换(MBT)器件，目前还没有关于MBT的报道。在本文中，我们通过在无氧铜衬底上使用精密金刚石切割进行模具制作和PDMS复制，成功地制作了MBT器件。该装置的混合长度为10.4 mm，能在51ms内实现FITC溶液(D=2.6×10(-10)m(2)S(-1))和免疫球蛋白G溶液(D=4.6×10(-11)m(2)S(-1))在306ms内完全混合。与无MBT结构的微通道相比，FITC溶液的混合速度提高了70倍，IgG溶液的混合速度提高了900倍。在MBT器件中实现完全混合的Péclet数估计为6.9×10(4)。
We developed a new passive-type micromixer based on the baker's transformation and realized a fast mixing of a protein solution, which has lower diffusion constant. The baker's transformation is an ideal mixing method, but there is no report on the microfluidic baker's transformation (MBT), since it is required to fabricate the complicated three-dimensional (3D) structure to realize the MBT device. In this note, we successfully fabricate the MBT device by using precision diamond cutting of an oxygen-free copper substrate for the mould fabrication and PDMS replication. The MBT device with 10.4 mm mixing length enables us to achieve complete mixing of a FITC solution (D = 2.6 × 10(-10) m(2) s(-1)) within 51 ms and an IgG solution (D = 4.6 × 10(-11) m(2) s(-1)) within 306 ms. Its mixing speed is 70-fold higher for a FITC solution and 900-fold higher for an IgG solution than the mixing speed by the microchannel without MBT structures. The Péclet number to attain complete mixing in the MBT device is estimated to be 6.9 × 10(4).