Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches
Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches
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DOI：
10.1074/jbc.m512118200
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发表时间：
2006-04-21
影响因子：
4.8
通讯作者：
Gordon, JI
中科院分区：
文献类型：
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作者：
Giannakis, M;Stappenbeck, TS;Gordon, JI
我们已经对来自激光捕获显微切割的成年小鼠胃和小肠上皮祖细胞的36，641个表达序列标签进行了测序，分别获得了4031个和3324个独特的转录本。使用基因本体论（GO）术语，每个数据集进行了比较，从完整的成人胃和小肠的cDNA文库。针对在造血、神经和胚胎干细胞转录组中代表的GO类别中的基因过滤在祖细胞中富集的GO类别中的基因，并映射到转录因子网络上，加上典型的信号转导和代谢途径。Wnt/β-连环蛋白、磷酸肌醇-3/Akt激酶、胰岛素样生长因子-1、血管内皮生长因子、整联蛋白和γ-氨基丁酸受体信号传导级联，加上甘油脂质、脂肪酸和氨基酸代谢途径是在成年肠道祖细胞中显著代表的那些。结果显示，与其他干细胞群体相比，成体肠道干细胞具有共同和独特的特征。
We have sequenced 36,641 expressed sequence tags from laser capture microdissected adult mouse gastric and small intestinal epithelial progenitors, obtaining 4031 and 3324 unique transcripts, respectively. Using Gene Ontology ( GO) terms, each data set was compared with cDNA libraries from intact adult stomach and small intestine. Genes in GO categories enriched in progenitors were filtered against genes in GO categories represented in hematopoietic, neural, and embryonic stem cell transcriptomes and mapped onto transcription factor networks, plus canonical signal transduction and metabolic pathways. Wnt/beta-catenin, phosphoinositide-3/Akt kinase, insulin-like growth factor-1, vascular endothelial growth factor, integrin, and gamma-aminobutyric acid receptor signaling cascades, plus glycerolipid, fatty acid, and amino acid metabolic pathways are among those prominently represented in adult gut progenitors. The results reveal shared as well as distinctive features of adult gut stem cells when compared with other stem cell populations.