Luminescent immunodetection of western-blotted proteins from coomassie-stained polyacrylamide gel.
Luminescent immunodetection of western-blotted proteins from coomassie-stained polyacrylamide gel.
复制标题
对考马斯染色聚丙烯酰胺凝胶中的蛋白质印迹蛋白进行发光免疫检测。
DOI：
10.1016/0003-2697(89)90227-3
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发表时间：
1989
影响因子：
2.9
通讯作者：
Vachereau,A
中科院分区：
文献类型：
--
作者：
Vachereau,A
用辣根过氧化物酶标记的抗体对考马斯染色的硝酸纤维蛋白印迹进行免疫检测，可以用过氧化物酶底物鲁米诺快速有效地进行。产生的发光用放射自显影胶片检测。这一程序允许直接鉴定染色的硝酸纤维素片的免疫检测条带，而不使用放射性标记的二次抗体。由于该方法简便、灵敏，特别适合于免疫检测蛋白的纯化。
Immunodetection with horseradish peroxidaselinked antibodies on Coomassie-stained nitrocellulose blots can be performed efficiently and rapidly with the peroxidase substrate luminol. The luminescence produced is detected with radioautographic film. This procedure allows a direct identification of immunodetected bands of stained nitrocellulose sheets without using radiolabeled secondary antibodies. Because of its convenience and sensitivity, this method could be particularly suitable for purification of immunodetected proteins.