Preparation of primary microglia cultures from postnatal mouse and rat brains.
Preparation of primary microglia cultures from postnatal mouse and rat brains.
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DOI：
10.1007/978-1-62703-520-0_4
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发表时间：
2013-01-01
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影响因子：
--
通讯作者：
Deierborg, Tomas
中科院分区：
文献类型：
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作者：
Deierborg, Tomas
小胶质细胞是大脑的炎症细胞，在神经病理条件下被激活。为了研究小胶质细胞的生物学，这些细胞可以从大脑中分离出来，并从促炎性和抗炎性细胞因子的产生、炎症刺激下细胞内信号通路的参与、吞噬和其他几个生物学方面进行分析，以了解它们在大脑中的作用。在本书的这一章中，我将讨论小胶质细胞，并描述如何从出生后的小鼠和大鼠大脑中培养这些细胞。
Microglia are the inflammatory cells of the brain and are activated in neuropathological conditions. To study the biology of microglia, these cells can be isolated from the brain and analyzed in terms of pro- and anti-inflammatory cytokine production, involvement of intracellular signaling pathways upon inflammatory stimuli, phagocytosis, and several more biological aspects to understand their role in the brain. In this book chapter, I will discuss microglial cells and describe how these cells can be cultured from a postnatal mouse and rat brain.