EXTENDED ATTENUATION OF CORTICOSTRIATAL POWER AND COHERENCE AFTER ACUTE EXPOSURE TO VAPOURIZED Δ9 TETRAHYDROCANNABINOL IN RATS.
EXTENDED ATTENUATION OF CORTICOSTRIATAL POWER AND COHERENCE AFTER ACUTE EXPOSURE TO VAPOURIZED Δ9 TETRAHYDROCANNABINOL IN RATS.
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DOI：
10.1097/cxa.0000000000000063
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发表时间：
2019-09
影响因子：
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通讯作者：
T. F. Nelong;Bryan W Jenkins;M. Perreault;J. Khokhar
中科院分区：
文献类型：
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作者：
T. F. Nelong;Bryan W Jenkins;M. Perreault;J. Khokhar
超过14%的加拿大人使用大麻，其中近60%的人报告每天或每周使用大麻。吸入大麻蒸气最近越来越受欢迎，但这种暴露对神经活动的影响仍然未知。在这项研究中，我们评估了急性暴露于汽化的Δ9-四氢大麻酚（THC）对大鼠神经回路动力学的影响。目的研究急性暴露于四氢大麻酚（THC）后，大鼠背侧纹状体（dStr）、眶额皮质（OFC）和前额叶皮质（PFC）神经活动的变化。方法大鼠植入靶向dStr、OFC和PFC的电极阵列。大鼠使用火山®蒸发器给予THC（或溶剂），并在有机玻璃室中以交叉设计进行局部场电位记录，洗脱期为一周。结果THC雾化给药后，在γ范围（>32-100 Hz）内的dStr、OFC和PFC内观察到谱功率降低。在THC给药后7天，γ的大多数变化仍然存在。在OFC-PFC和dStr-PFC区域对之间也观察到伽马相干性降低。结论：单次暴露于汽化的THC会抑制皮质和背侧纹状体的伽马功率和相干性，这种影响似乎持续至少一周。考虑到伽马功能减退在精神分裂症中的作用，这些发现可能为THC已知的拟精神病作用提供机制见解。
Introduction Over 14% of Canadians use cannabis, with nearly 60% of these individuals reporting daily or weekly use. Inhalation of cannabis vapour has recently gained popularity, but the effects of this exposure on neural activity remain unknown. In this study, we assessed the impact of acute exposure to vapourized Δ9-tetrahydrocannabinol (THC) on neural circuit dynamics in rats. Objectives We aimed to characterize the changes in neural activity in the dorsal striatum (dStr), orbitofrontal cortex (OFC), and prefrontal cortex (PFC), after acute exposure to THC vapour. Methods Rats were implanted with electrode arrays targeting the dStr, OFC, and PFC. Rats were administered THC (or vehicle) using a Volcano® vapourizer and local field potential recordings were performed in a plexiglass chamber in a cross-over design with a week-long washout period. Results Decreased spectral power was observed within the dStr, OFC, and PFC in the gamma range (>32-100 Hz) following vapourized THC administration. Most changes in gamma were still present 7 days after THC administration. Decreased gamma coherence was also observed between the OFC-PFC and dStr-PFC region-pairs. Conclusion A single exposure to vapourized THC suppresses cortical and dorsal striatal gamma power and coherence, effects that appear to last at least a week. Given the role of gamma hypofunction in schizophrenia, these findings may provide mechanistic insights into the known psychotomimetic effects of THC.