2,3-Butanedione suppresses gray mold of postharvest fruit by activating the autophagy of Botrytis cinerea
2,3-Butanedione suppresses gray mold of postharvest fruit by activating the autophagy of Botrytis cinerea
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DOI：
10.1016/j.postharvbio.2022.112057
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发表时间：
2022-08-04
影响因子：
7
通讯作者：
Tian, Shiping
中科院分区：
文献类型：
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作者：
Li, Guangjin;Chen, Yong;Tian, Shiping
植物和微生物产生的挥发性有机化合物因具有很强的抑菌作用而被广泛应用于采后病害的防治。研究了柠檬酸代谢产物2，3-丁二酮对采后灰霉病菌的抑制作用。2，3-丁二酮处理对B的菌落生长、孢子萌发率和芽管长度均有明显的抑制作用。在人工接种和自然接种条件下，均能有效地减轻接种果的病害发生。转录组分析显示，2，3-丁二酮处理导致精氨酸生物合成相关基因、核糖体生物合成相关基因以及Bcpg 1 -3、BcNEP 1、Bcboa 2和Bmpl等致病相关基因的表达下调。此外，处理导致与蛋白水解、过氧化物酶体和自噬相关的基因表达增加。其中，自噬途径最为突出，该途径中的关键基因（BcATGs和BcVPS 45）被2，3-丁二酮处理激活。此外，在2，3-丁二酮处理的真菌细胞中观察到许多含有细胞质成分的自噬空泡。结果表明，2，3-丁二酮不仅通过干扰B中真菌生长和致病性相关基因的表达来抑制采后灰霉病的发生。灰霉病，但也诱导自噬活性。这些结果为阐明丁二酮对B的抑菌机理提供了理论依据。灰叶
The volatile organic compounds produced by plants and microorganisms have been widely used for postharvest disease control due to their high antifungal capacity. In this research, a volatile product of citrate metabolism, 2,3-butanedione, was assayed against gray mold caused by Botrytis cinerea on postharvest fruit. 2,3-Butanedione treatment exhibited a distinct inhibitory effect on colony growth, conidial germination rate and germ tube length of B. cinerea in vitro and efficiently mitigated the disease prevalence of inoculated fruit under both artificial and natural inoculation conditions. Transcriptome analysis displayed that 2,3-butanedione treatment led to down-regulated expression of genes associated with arginine biosynthesis, ribosome biogenesis and several pathogenicity-related genes, including Bcpg1-3, BcNEP1, Bcboa2 and Bmpl. Furthermore, treatment led to increased genes expression related to proteolysis, peroxisome and autophagy. Among them, the autophagy pathway was most prominent, and key genes in this pathway (BcATGs and BcVPS45) were activated by 2,3-butanedione treatment. Moreover, numerous autophagic vacuoles containing cytoplasmic components were observed in the 2,3-butanedione-treated fungal cells. These results suggest that 2,3-butanedione inhibits gray mold on postharvest fruit by not only interfering the gene expression of fungal growth and pathogenicity in B. cinerea, but also inducing autophagic activity. Collectively, these results provide a theoretical basis for elucidating the underlying antifungal mechanism of 2,3-butanedione against B. cinerea.