COMPARISON OF EPIDERMAL GROWTH-FACTOR BINDING AND RECEPTOR DISTRIBUTION IN NORMAL HUMAN-EPIDERMIS AND EPIDERMAL APPENDAGES
COMPARISON OF EPIDERMAL GROWTH-FACTOR BINDING AND RECEPTOR DISTRIBUTION IN NORMAL HUMAN-EPIDERMIS AND EPIDERMAL APPENDAGES
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DOI：
10.1111/1523-1747.ep12264708
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发表时间：
1984-01-01
影响因子：
6.5
通讯作者：
KING, LE
中科院分区：
文献类型：
--
作者：
NANNEY, LB;MAGID, M;KING, LE
用2种不同的光镜方法对正常人表皮和其他皮肤结构中的表皮生长因子受体进行定位。通过将完整皮肤标本与[125I]EGF孵育、组织切片和放射自显影，研究了EGF与EGF受体胞外部分的结合。采用两步间接免疫细胞化学法(辣根过氧化物酶)和洗涤剂通透性组织，用单特异性抗EGF受体抗体对EGF受体分子(IR-EGF/R)进行定位。后一种方法测量了总的EGF受体池：占据和/或内化的形式、前体形式和部分降解的保留免疫反应性的EGF受体形式。[125I]EGF/R和IR-EGF/R定位研究均表明，EGF受体存在于毛囊中的基底层角质形成细胞、皮脂细胞、外根鞘细胞、直毛肌的平滑肌细胞和真皮动脉中。[~(125)I]EGF/R和IR-EGF/R在汗腺真皮导管中含量最高。[~(125)I]EGF/R和IR-EGF/R的分布与EGF仅参与正常人表皮及其附件细胞分裂和增殖的观点不一致，即EGF受体也存在于不快速增殖的组织中。表皮生长因子在皮肤中的调节作用可能比人们之前认为的更复杂。
To localize epidermal growth factor (EGF) receptors in normal human epidermis and other skin structures, 2 different light microscopic methods were used. EGF binding ([125I]EGF/R) to the extracellular portion of the EGF receptor was studied by incubating intact skin samples with [125I]EGF, sectioning the tissues and performing autoradiography. Immunoreactive EGF receptor molecules (IR-EGF/R) were localized with a monospecific anti-EGF receptor antibody using a 2-step indirect immunocytochemical method (horseradish peroxidase) and detergent permeabilized tissues. This latter method measured the total pool of EGF receptors: occupied and/or internalized forms, precursor forms and partially degraded forms of the EGF receptor that retain immunoreactivity. Both the [125I]EGF/R and IR-EGF/R localization studies indicated that EGF receptors were present in basal epidermal keratinocytes, sebocytes, outer root sheath cells in hair follicles, smooth muscle cells of arrector pili muscles, and dermal arteries. The highest levels of [125I]EGF/R and IR-EGF/R were found in the dermal ducts of eccrine sweat glands. The distribution of both [125I]EGF/R and IR-EGF/R was not consistent with the concept that EGF exclusively is involved in cellular division and proliferation in normal human epidermis and its appendages, i.e., EGF receptors were also found in tissues that do not undergo rapid proliferation. EGF may have a more complex regulatory role in the skin than was previously thought.