cDNA-AFLP analysis of inducible gene expression in zinc hyperaccumulator Sedum alfredii Hance under zinc induction
cDNA-AFLP analysis of inducible gene expression in zinc hyperaccumulator Sedum alfredii Hance under zinc induction
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DOI：
10.1016/j.envexpbot.2009.11.013
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发表时间：
2010-04-01
影响因子：
5.7
通讯作者：
Islam, Ejazul
中科院分区：
文献类型：
--
作者：
Chao, Yue-en;Zhang, Min;Islam, Ejazul
东南景天Sedum alfredii Hance（S. alfredii）是我国重金属污染区的一种锌超积累植物。本研究采用cDNA扩增片段长度多态性（cDNA-AFLP）技术，对超积累型S. alfredii与非超积累S.在水培条件下，用Zn处理的alfredii。超富集植物和非超富集植物处理时间均为0 h。分别用500 μ M Zn（NO_3）（2）和50 μ M Zn（NO_3）（2）处理24 h和8 d。在超积累S. alfredii与非超积累植物相比。16个TDFs在NCBI数据库中具有显著匹配，6个TDFs没有显著匹配。此外，还克隆了两种生态型的肌动蛋白基因的全长cDNA序列。通过RT-PCR和RACE技术，对超积累S. alfredii进行了基因表达分析。alfredii实时荧光定量PCR。(C)2009爱思唯尔有限公司版权所有。
Sedum alfredii Hance (S. alfredii) has been identified as a zinc hyperaccumulating plant species from heavy metal polluted areas of China. In this study, a cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis was employed to identify differentially expressed genes of hyperaccumulating S. alfredii compared with non-hyperaccumulating S. alfredii, under Zn treatment in hydroponic culture. Both the hyperaccumulator and non-hyperaccumulator were treated for 0 h. 24 h and 8 d with 500 mu M Zn(NO3)(2) and 50 mu M Zn(NO3)(2), respectively. Twenty-two transcript-derived fragments (TDFs) were identified as Zn responsive fragments in hyperaccumulating S. alfredii compared with the non-hyperaccumulator. Sixteen TDFs had significant matches in the NCBI database and six TDFs had no significant matches. In addition, the full-length cDNA sequences of the actin gene were cloned from the two ecotypes of S. alfredii by RT-PCR and RACE, and used as an internal standard for the confirmation of gene expression in hyperaccumulating S. alfredii by real-time PCR. (C) 2009 Elsevier B.V. All rights reserved.