LACK OF N-REGIONS IN ANTIGEN RECEPTOR VARIABLE REGION GENES OF TDT-DEFICIENT LYMPHOCYTES
LACK OF N-REGIONS IN ANTIGEN RECEPTOR VARIABLE REGION GENES OF TDT-DEFICIENT LYMPHOCYTES
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DOI：
10.1126/science.8356451
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发表时间：
1993-08-27
期刊：
影响因子：
56.9
通讯作者：
ALT, FW
中科院分区：
文献类型：
--
作者：
KOMORI, T;OKADA, A;ALT, FW
在来自可变（V）、多样性（D）和连接（J）区段的免疫球蛋白和T细胞受体可变区基因的组装期间，种系编码的库通过包括在基因区段连接处的核苷酸（N区）的模板非依赖性添加的过程而进一步多样化。末端脱氧核苷酸转移酶（TdT）缺陷的淋巴细胞的可变区基因中没有N区，这表明TdT负责N区的添加。此外，某些可变区基因出现在TdT缺陷的胸腺细胞中的频率增加，这表明N区的加入也通过减轻特定V，D和J片段的连接上施加的序列特异性约束来影响库的发展。
During the assembly of immunoglobulin and T cell receptor variable region genes from variable (V), diversity (D), and joining (J) segments, the germline-encoded repertoire is further diversified by processes that include the template-independent addition of nucleotides (N regions) at gene segment junctions. Terminal deoxynucleotidyl transferase (TdT)-deficient lymphocytes had no N regions in their variable region genes, which shows that TdT is responsible for N region addition. In addition, certain variable region genes appeared at increased frequency in TdT-deficient thymocytes, which indicates that N region addition also influences repertoire development by alleviating sequence-specific constraints imposed on the joining of particular V, D, and J segments.