A lymphocyte homing receptor (L-selectin) mediates the in vitro attachment of lymphocytes to myelinated tracts of the central nervous system.
A lymphocyte homing receptor (L-selectin) mediates the in vitro attachment of lymphocytes to myelinated tracts of the central nervous system.
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淋巴细胞归巢受体（L-选择素）在体外介导淋巴细胞与中枢神经系统有髓鞘的附着。
DOI：
10.1172/jci115498
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Rosen,SD
中科院分区：
文献类型：
--
作者：
Huang,K;Geoffroy,JS;Singer,MS;Rosen,SD
淋巴细胞首先通过黏附高内皮微静脉进入淋巴结，这是一种由凝集素样淋巴细胞受体(L-选择素)介导的黏附事件。此前，体外试验表明，淋巴细胞优先附着在脑组织切片中富含髓磷脂的区域。在这里，使用重组形式的L-选择素作为免疫组织化学试剂，我们展示了L-选择素在中枢有髓区域的潜在配体，而不是外周神经系统。利用几种抗体和佛波酯对该受体的下调，我们证实人淋巴细胞上的L-选择素主要参与了淋巴细胞对有髓区域的黏附。在小鼠淋巴细胞上，L-选择素的贡献似乎是部分的。这些发现提出了这样一种可能性，即白细胞通过L-选择素依赖机制靶向富髓磷脂区域，可能是某些中枢神经系统脱髓鞘疾病发病机制的一个因素。
Lymphocytes enter lymph nodes by first adhering to high endothelial venules, an adhesive event mediated by a lectinlike lymphocyte receptor (L-selectin). Previously, it was shown with an in vitro assay that lymphocytes preferentially adhere to myelin-rich regions in brain sections. Here, using a recombinant form of L-selectin as an immunohistochemical reagent, we demonstrate potential ligands for L-selectin in myelinated regions of the central but not the peripheral nervous system. Using several antibodies and phorbol ester downmodulation of the receptor, we establish that L-selectin on human lymphocytes has a primary involvement in lymphocyte adherence to the myelinated regions. On mouse lymphocytes, the contribution of L-selectin appears to be partial. These findings raise the possibility that leukocyte targeting to myelin-rich regions, via a L-selectin dependent mechanism, may be a factor in the pathogenesis of certain central nervous system demyelinating diseases.Images