Tenascin C may regulate the recruitment of smooth muscle cells during coronary artery development
Tenascin C may regulate the recruitment of smooth muscle cells during coronary artery development
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DOI：
10.1016/j.diff.2011.03.002
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发表时间：
2011-06-01
期刊：
影响因子：
2.9
通讯作者：
Nakajima, Yuji
中科院分区：
文献类型：
--
作者：
Ando, Katsumi;Takahashi, Makiko;Nakajima, Yuji
腱生蛋白C（TNC）是一种细胞外糖蛋白，被认为参与器官发生和再生过程中的组织重塑。利用鸡胚心脏，我们研究了TNC在近端冠状动脉形成过程中的时空表达模式。免疫组化显示TNC沉积在发育中的冠状动脉干周围，并且TNC与血管平滑肌α-肌动蛋白共定位。鹌鹑前心外膜器官（PEO）移植到Aquail-鸡嵌合体中，结果表明鹌鹑组织来源的细胞参与了近端冠状动脉内皮细胞和壁细胞的建立，鹌鹑组织来源的壁细胞显示TNC。在TNC中培养的前心外膜细胞显示肌纤维母细胞/平滑肌细胞表型，中和性抗TNC抗体抑制平滑肌标志物的表达。这些观察结果表明TNC在新生近端冠状动脉的壁平滑肌发育中发挥作用。(C)2011年国际分化学会。由爱思唯尔有限公司出版。保留所有权利。
Tenascin C (TNC) is an extracellular glycoprotein that is thought to be involved in tissue remodeling during organogenesis and regeneration. Using avian embryonic hearts, we investigated the spatio-temporal expression patterns of TNC during the formation of the proximal coronary artery. Immunohistochemistry showed that TNC was deposited around the developing coronary stem and that TNC colocalized with vascular smooth muscle alpha-actin. Aquail-chick chimera, in which a quail proepicardial organ (PEO) had been transplanted, showed that quail tissue-derived cells contributed to the establishment of the endothelial and mural cells of the proximal coronary artery, and the quail tissue-derived mural cells displayed TNC. Proepicardial cells cultured in TNC showed the myofibro-blast/smooth muscle cell phenotype and neutralizing anti-TNC antibody suppressed the expression of smooth muscle markers. These observations suggest that TNC plays a role in the mural smooth muscle development of the nascent proximal coronary artery. (C) 2011 International Society of Differentiation. Published by Elsevier Ltd. All rights reserved.