Time-course of GDNF and its receptor expression after brain injury in the rat
Time-course of GDNF and its receptor expression after brain injury in the rat
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DOI：
10.1016/j.neulet.2008.04.089
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发表时间：
2008-07-04
影响因子：
2.5
通讯作者：
Mudo, Giuseppa
中科院分区：
文献类型：
--
作者：
Cheng, Qingzhang;Di Liberto, Valentina;Mudo, Giuseppa
本工作的目的是通过原位杂交，对两种大鼠脑损伤模型中胶质细胞源性神经营养因子（GDNF）及其受体 mRNA 表达进行时程分析：（a）海马结构注射鹅膏蕈酸引起的兴奋性毒性损伤； (b) 针插入大脑皮层（包括胼胝体白质）造成的机械损伤。时程分析（从 6 小时到 8 天）显示，两种病变模型中 GDNF 及其受体（RET、GFR α-1 和 GFR α-2）mRNA 表达均有差异上调。 GDNF 及其受体 mRNA 表达的体内调节表明它们参与脑损伤后发生的神经元保护和再生过程。 (c) 2008 Elsevier Ireland Ltd. 保留所有权利。
The aim of the present work was to perform, by in situ hybridization, a time-course analysis of the glial cell line-derived neurotrophic factor (GDNF) and its receptor mRNA expression in two models of brain injury in the rat: (a) excitotoxic lesion by ibotenic acid injection in the hippocampal formation; (b) mechanical lesion by needle insertion through the cerebral cortex including the white matter of the corpus callosum. The time-course analysis, ranging from 6 h to 8 days, showed that the GDNF and its receptor (RET, GFR alpha-1 and GFR alpha-2) mRNA expressions were differentially up-regulated in both models of lesion. This in vivo regulation of the GDNF and its receptor mRNA expression indicates their involvement in the process of neuronal protection and regeneration occurring after brain injury. (c) 2008 Elsevier Ireland Ltd. All rights reserved.