The occurrence of geometric polyprenol isomers in the rubber-producing plant, Eucommia ulmoides Oliver
The occurrence of geometric polyprenol isomers in the rubber-producing plant, Eucommia ulmoides Oliver
复制标题
DOI：
10.1007/s11745-001-0778-5
复制
发表时间：
2001-07-01
期刊：
影响因子：
1.9
通讯作者：
Kobayashi, A
中科院分区：
文献类型：
--
作者：
Bamba, T;Fukusaki, E;Kobayashi, A
采用超临界流体色谱法对杜仲奥利弗中聚戊烯醇的链长和几何异构体进行了分析。经过大量的努力，建立分离条件的几何异构体，苯基键合硅胶填充柱，干净地分离聚反式和顺式异戊二烯醇。首次在植物中证实了长链聚反式异戊二烯醇（> 9聚体）的存在。反式异构体在叶、种皮和根中发现，但在树皮和种子中没有发现。该植物中的聚反式异戊二烯醇可能作为反式聚异戊二烯生物合成的中间体。
The chain length and geometric isomerism of polyprenols from Eucommia ulmoides Oliver were analyzed using supercritical fluid chromatography. After intensive effort to establish separation conditions for geometric isomers, a phenyl-bonded silica gel-packed column was found that cleanly separated poly-trans and -cis prenols. The presence of long-chain poly-trans prenols (> 9 mers) was confirmed for the first time in plants. Trans isomers were found in the leaf, seed coat, and root, but not in the bark and seed. Poly-trans prenols in this plant may act as intermediates for trans-polyisoprene biosynthesis.