ANTIBODIES TO BREVETOXIN-B - SEROLOGIC DIFFERENTIATION OF BREVETOXIN-B AND BREVETOXIN-A
ANTIBODIES TO BREVETOXIN-B - SEROLOGIC DIFFERENTIATION OF BREVETOXIN-B AND BREVETOXIN-A
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DOI：
10.1016/0041-0101(92)90537-f
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发表时间：
1992-04-01
期刊：
影响因子：
2.8
通讯作者：
SHIMIZU, Y
中科院分区：
文献类型：
--
作者：
LEVINE, L;SHIMIZU, Y
将短杆菌毒素B与牛白蛋白的氨基反应，然后用硼氢化钠处理，制备短杆菌毒素B免疫原。免疫兔产生的抗体结合[H-3]短杆菌毒素B-醇。这种结合随着重复免疫而增加（在三个免疫疗程后，一只兔的K（D）约为10 pM）。Brevetoxin B的抑制作用比Brevetoxin A强500倍以上。当在竞争性结合试验中用[H-3]短杆菌毒素B-醇-短杆菌毒素B抗体进行测试时，醇作为抑制剂的有效性是短杆菌毒素B的6倍。尽管在结构上与短毒素A有一些相似性，但短毒素B的抗体不识别短毒素A的主链。
A brevetoxin B immunogen was prepared by reacting brevetoxin B with the amino groups of bovine albumin followed by treatment with sodium borohydride. Immunized rabbits produced antibodies that bound [H-3]brevetoxin B-alcohol. This binding increased with repeated immunization (K(D) for one rabbit after three courses of immunization was about 10 pM). Brevetoxin B inhibited greater than 500 times more effectively than brevetoxin A. When tested in a competitive binding assay with [H-3]brevetoxin B-alcohol-brevetoxin B antibody, the alcohol was six times more effective as an inhibitor than brevetoxin B. Despite some similarities in structure with brevetoxin A, the antibodies to brevetoxin B did not recognize the backbone of brevetoxin A.