Functional annotation of a full-length Arabidopsis cDNA collection
Functional annotation of a full-length Arabidopsis cDNA collection
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DOI：
10.1126/science.1071006
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发表时间：
2002-04-05
期刊：
影响因子：
56.9
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
--
作者：
Seki, M;Narusaka, M;Shinozaki, K
全长互补DNA对于基因组序列的正确注释和基因及其产物的功能分析是必不可少的。我们分离了155,144个RIKEN拟南芥全长(RAFL)的cDNA克隆。155,144个raf1基因的3‘端表达序列标签(EST)被聚为14,668个非冗余的cDNA组，约占预测基因的60%。我们还从14,034个非冗余的cDNA组中获得了5‘EST，并构建了启动子数据库。RAFL的cDNA序列数据库对于启动子分析和预测的转录单位和基因产物的正确注释是有用的。此外，全长cDNA是分析植物蛋白表达谱、功能和结构的有用资源。
Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.