Isothermal assembly of DNA origami structures using denaturing agents
Isothermal assembly of DNA origami structures using denaturing agents
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DOI：
10.1021/ja8030196
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发表时间：
2008-08-06
影响因子：
15
通讯作者：
Simmel, Friedrich C.
中科院分区：
文献类型：
--
作者：
Jungmann, Ralf;Liedl, Tim;Simmel, Friedrich C.
DNA折纸是DNA自组装领域最有前途的最新发展之一。它允许通过使用大量的短“钉”链折叠长的病毒支架链来构建任意纳米级的图案和物体。组装通常是通过DNA分子的热退火作为缓冲溶液来完成的。在这里，我们证明了2D和3D折纸结构都可以通过在变性缓冲液中加热DNA链，然后控制降低变性剂浓度来等温组装。这为集成温度敏感组件打开了折纸组装的大门。
DNA origami is one the most promising recent developments in DNA self-assembly. It allows for the construction of arbitrary nanoscale patterns and objects by folding a long viral scaffold strand using a large number os short "staple" strands. Assembly is usually accomplished by thermal annealing of DNA molecules is buffer solution. We here demonstrate that both 2D and 3D origami structures can be assembled isothermally by annealing the DNA strands in denaturing buffer, followed by a controlled reduction of denaturant concentration. This opens up origami assembly for the integration of temperature-sensitive components.