Transcriptome Analysis Reveals the Regulation Role of miR-144-5p in Intestinal Immunity of Japanese Flounder (Paralichthys olivaceus)
Transcriptome Analysis Reveals the Regulation Role of miR-144-5p in Intestinal Immunity of Japanese Flounder (Paralichthys olivaceus)
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DOI：
10.1007/s11802-023-5378-y
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发表时间：
2023-05
影响因子：
1.6
通讯作者：
Yingrui Li;Lin Su;Baoshan Guo;Kefeng Xu;Shun Zhou;Yunji Xiu
中科院分区：
文献类型：
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作者：
Yingrui Li;Lin Su;Baoshan Guo;Kefeng Xu;Shun Zhou;Yunji Xiu
MicroRNA（miRNAs）是一种22个核苷酸长度的微管理分子，可指导多种靶基因的转录后调控，理论上可作为炎症反应的诊断或治疗靶点。在鱼类中，miR-144- 5 p的表达在不同的细菌感染中有很大的差异，并且可以调节免疫相关基因以减少炎症的发生。本研究以牙鲆（Paralichthys olivaceus）为实验动物，研究miR-144- 5 p对肠道天然免疫的调节作用。比目鱼被2 μg g− 1 miR-144- 5 p阿克罗米干扰，并在注射后12小时从鱼中收获它们的组织（肠、肝和脾）。收集了超过6000万个高质量的读数。在miR-144- 5 p或miR-NC干扰后24小时，与对照组相比，分别鉴定出总共2704个和1823个差异表达基因（DEG）。根据GO和KEGG分析，DEG在多种免疫相关信号通路中富集，包括NOD样受体、Wnt和Toll样受体信号通路。使用KEGG分析，共发现了503个参与33个免疫相关信号通路的高度相互作用的DEG。此外，通过蛋白质相互作用网络发现了5个枢纽基因，构成了一个复杂的免疫调节网络。同时，这些枢纽基因主要参与黏着斑、Wnt信号通路以及肠道免疫网络中的IgT（伊加）产生通路。总之，miR-144- 5 p的缺失可以影响免疫相关基因和下游信号通路。我们的研究结果表明，miR-144- 5 p是与肠道先天免疫相关的基因网络和信号通路的调节剂。
MicroRNAs (miRNAs), 22-nucleotide-long micromanagers that guide the post-transcriptional regulation of a wide range of target genes, can theoretically be used as a diagnostic or therapeutic target for inflammatory reaction. In fish, miR-144-5p expression varies dramatically in response to the different bacterial infections and can regulate immunity-related genes to reduce the occurrence of inflammation. In this research, the regulation function of miR-144-5p to the intestinal innate immunity was udied in flounderParalichthys olivaceus. The flounders were interfered by 2 µg g−1miR-144-5p antagomir and their tissues (intestine, liver and spleen) were harvested from the fish at 12 h post-injection. More than 60 million high-quality reads were collected. At 24 hours after miR-144-5p or miR-NC interference, a total of 2704 and 1823 different-expresion genes (DEGs) were identified in comparison with control group, respectively. The DEGs were enriched in a variety of immunity-related signaling pathways, including NOD-like receptor, Wnt and Toll-like receptor signaling pathways, according to GO and KEGG analyses. A total of 503 highly interacting DEGs engaged in 33 immunity-related signaling pathways were discovered using KEGG analyses. Additionally, 5 hub genes were found by protein-protein interaction networks, which formed an intricate immune regulation network. Meanwhile, these hub genes were mostly involved in focal adhesion, Wnt signaling pathway, as well as the Intestinal Immune Network for IgT (IgA) Production Pathway. In conclusion, the loss of miR-144-5p can affect immunity-related genes and downstream signaling pathways. Our findings suggest that miR-144-5p is a modulator of gene networks and signaling pathways associated with intestinal innate immunity.