Chloroplast-encoded PsbT is required for efficient biogenesis of photosystem II complex in the green alga Chlamydomonas reinhardtii
Chloroplast-encoded PsbT is required for efficient biogenesis of photosystem II complex in the green alga Chlamydomonas reinhardtii
复制标题
DOI：
10.1007/s11120-008-9344-8
复制
发表时间：
2008-10-01
影响因子：
3.7
通讯作者：
Takahashi, Yuichiro
中科院分区：
文献类型：
--
作者：
Ohnishi, Norikazu;Takahashi, Yuichiro
小分子疏水性多肽PsbT与光系统II(PSII)反应中心(d1/d2异源二聚体)有关。在此，我们报道了在绿藻衣藻Y-1突变体光诱导绿化过程中，PsbT的缺失对PSII复合体生物发生的影响。Y-1不能在黑暗中合成叶绿素，但在光下合成。暗培养的y-1细胞没有积累主要的PSII蛋白，但积累了少量的PsbT。光照后，PsbT立即合成，而叶绿素、主要的PSII蛋白和放氧活性在1h后增加。不加PsbT的y-1细胞叶绿素和PSI蛋白的积累速度相似，而PSII复合体的积累在绿色化过程中表现出明显的延缓。PsbT的缺失不影响PSII蛋白的合成。这些结果表明，PsbT是PSII复合体高效生物发生所必需的。
The small hydrophobic polypeptide PsbT is associated with the photosystem II (PSII) reaction center (D1/D2 heterodimer). Here, we report the effect of the deletion of PsbT on the biogenesis of PSII complex during light-induced greening of y-1 mutants of the green alga Chlamydomonas reinhardtii. The y-1 is unable to synthesize chlorophylls in the dark but do so in the light. The dark-grown y-1 cells accumulated no major PSII proteins but a small amount of PsbT. Upon illumination, PsbT was immediately synthesized while chlorophylls, major PSII proteins, and O-2-evolving activity increased after a 1-h lag. The y-1 cells without PsbT accumulated chlorophylls and PSI protein at a similar rate, whereas the accumulation of PSII complex was specifically retarded during greening. The absence of PsbT did not affect the synthesis of PSII proteins. These results indicate that PsbT is required for the efficient biogenesis of PSII complex.