Flavonol-containing phosphorylated pullulan may attenuate pulp inflammation
Flavonol-containing phosphorylated pullulan may attenuate pulp inflammation
复制标题
含黄酮醇的磷酸化普鲁兰多糖可以减轻牙髓炎症
DOI：
10.1111/j.1365-2591.2012.02095.x
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
J. Yonehiro
中科院分区：
文献类型：
--
作者：
K.Kishihara;M.Naito;Y.Okahata;J. Yonehiro
目的利用已建立的牙髓炎模型，寻找可能的试剂来减少炎症反应，以开发新的牙髓覆盖材料，并测试磷酸化普鲁兰作为这种抗炎试剂的载体的可能性。方法采用transwell系统进行共培养。通过测量细胞产生的细胞因子来评估炎症反应。我们测试了两种黄酮类化合物木犀草素和槲皮素的抗炎作用，以及磷酸化普鲁兰的抗炎作用。磷酸化普鲁兰可能与羟基磷灰石形成足够的边缘密封，并缓慢释放作为黄酮类化合物载体的木犀草素。结果黄酮醇，尤其是木犀草素，能显著降低炎症细胞因子的产生，并通过共培养而增强。木犀草素被磷酸化的普鲁兰成功包裹。最后，通过共培养证实磷酸化普鲁兰释放的木犀草素能有效减少细胞因子的产生。结论磷酸化普鲁兰与木犀草素联用治疗牙髓炎症有较好的应用前景。
AimTo find possible reagents to minimize inflammatory responses by using an established pulpitis models for the purpose of developing new pulp‐capping materials, and to test the possible use of phosphorylated pullulan as a carrier for such an anti‐inflammatory reagent.MethodologyCo‐culturing was performed using transwell systems. Inflammatory responses were evaluated by measuring cytokines produced by the cells. The effects of two flavonoids, luteolin and quercetin, as anti‐inflammatory reagents, and phosphorylated pullulan, which potentially achieves a sufficient marginal sealing to hydroxyapatite and slowly releases luteolin, as a carrier for flavonoids, were tested.ResultsFlavonols, particularly luteolin, dramatically attenuated inflammatory cytokine production, which was augmented by co‐cultures. Luteolin was successfully enclosed by phosphorylated pullulan. Finally, it was confirmed that luteolin released from phosphorylated pullulan was effective in reducing cytokine production by co‐cultures.ConclusionsCombination of phosphorylated pullulan and luteolin could be potentially used in the treatment of dental pulp inflammation.