Establishment of Intestinal Epithelial Cell Lines from Adult Mouse Small and Large Intestinal Crypts
Establishment of Intestinal Epithelial Cell Lines from Adult Mouse Small and Large Intestinal Crypts
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DOI：
10.1271/bbb.100887
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发表时间：
2011-05-01
影响因子：
1.6
通讯作者：
Totsuka, Mamoru
中科院分区：
文献类型：
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作者：
Iwamoto, Taku;Yamada, Kiyoshi;Totsuka, Mamoru
从成年小鼠肠隐窝中建立小肠上皮细胞系和大肠上皮细胞系。建立的小型和大型IECs系（分别命名为aMoS7和aMoC1）均表达上皮标志物。与从成年小鼠肠道中分离的IECs类似，干扰素- γ处理在两种建立的细胞系中诱导了主要组织相容性复合体（MHC） II类分子的表达。MHC II类分子在小肠aMoS7细胞中的表达高于大肠aMoC1细胞。脂多糖和toll样受体1、2、3和7配体处理诱导两种成年IEC细胞系分泌白介素-6。这些结果表明，aMoS7和aMoC1细胞系可以作为分析IECs免疫功能的有用工具，特别是研究IECs对微生物组分的反应及其抗原递呈能力。
Small and large intestinal epithelial cell (IEC) lines were established from adult murine intestinal crypts. Both established small and large IECs line (named aMoS7 and aMoC1 respectively) expressed epithelial markers. Similarly to IECs isolated from adult mouse intestines, the expression of major histocompatibility complex (MHC) class II molecules was induced by interferon-gamma-treatment in both established cell lines. This expression of MHC class II molecules was higher in small intestinal aMoS7 cells than in large intestinal aMoC1 cells. Treatment with lipopolysaccharide and with ligands of Toll-like receptors 1, 2, 3, and 7 induced secretion of interleukin-6 from both adult IEC lines. These results suggest that the aMoS7 and aMoC1 cell lines can serve as useful tools in analyzing the immunological functions of IECs, especially in studying the IEC response to microbial components and its antigen presenting ability.