Gene switching in Amoeba proteus caused by endosymbiotic bacteria
Gene switching in Amoeba proteus caused by endosymbiotic bacteria
复制标题
内共生细菌引起的变形虫基因转换
DOI：
10.1242/jcs.00894
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发表时间：
2004
影响因子：
4
通讯作者：
K. Jeon
中科院分区：
文献类型：
--
作者：
T. Jeon;K. Jeon
在无共生体（D）和携带共生体（xD）的阿米巴菌株中研究了S-腺苷甲硫氨酸合成酶（SAMS）基因的表达，该合成酶催化细胞中主要甲基供体S-腺苷甲硫氨酸（AdoMet）的合成，以确定细菌内共生体的影响。共生体抑制宿主xD阿米巴中基因的表达，但阿米巴仍然表现出约一半的酶活性，发现在共生体自由D阿米巴。该研究旨在阐明阿米巴基因的抑制机制，并确定基因产物的替代来源。出乎意料的是，我们在变形虫中发现了第二个sams（sams 2）基因，它编码390个氨基酸。测量D和xD变形虫中SAMS活性和α-Met量的实验结果表明，在xD变形虫中发现的一半SAMS活性来自变形虫的SAMS 2，而不是来自它们的内共生体。变形虫sams基因的表达从sams 1切换到sams 2作为感染X-细菌的结果，提高了细菌sams基因表达的切换在共生关系的发展和宿主-病原体相互作用中发挥作用的可能性。这是第一份报告显示，这种开关在宿主sams基因的表达感染细菌。
The expression of genes for S-adenosylmethionine synthetase (SAMS), which catalyzes the synthesis of S-adenosylmethionine (AdoMet), a major methyl donor in cells, was studied in symbiont-free (D) and symbiont-bearing (xD) amoeba strains to determine the effect of bacterial endosymbionts. The symbionts suppressed the expression of the gene in host xD amoebae, but amoebae still exhibited about half the enzyme activity found in symbiont-free D amoebae. The study was aimed at elucidating mechanisms of the suppression of the amoeba's gene and determining the alternative source for the gene product. Unexpectedly, we found a second sams (sams2) gene in amoebae, which encoded 390 amino acids. Results of experiments measuring SAMS activities and amounts of AdoMet in D and xD amoebae showed that the half SAMS activity found in xD amoebae came from the amoeba's SAMS2 and not from their endosymbionts. The expression of amoeba sams genes was switched from sams1 to sams2 as a result of infection with X-bacteria, raising the possibility that the switch in the expression of sams genes by bacteria plays a role in the development of symbiosis and the host-pathogen interactions. This is the first report showing such a switch in the expression of host sams genes by infecting bacteria.
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影响因子：
2.9
作者：
McQueney, MS;Anderson, KS;Markham, GD
通讯作者：
Markham, GD
影响因子：
2.9
作者：
Takusagawa, F;Kamitori, S;Markham, GD
通讯作者：
Markham, GD
影响因子：
11.2
作者：
Jiaxin Cai;Z. Mao;J. Hwang;Shelly C. Lu
通讯作者：
Jiaxin Cai;Z. Mao;J. Hwang;Shelly C. Lu
DOI：
10.1152/ajpgi.1998.275.1.g14
发表时间：
1998-07-01
影响因子：
4.5
作者：
Huang, ZZ;Mao, ZB;Lu, SC
通讯作者：
Lu, SC
大肠杆菌中 S-腺苷甲硫氨酸合成酶的结构基因 metK 的序列。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Markham,GD;DeParasis,J;Gatmaitan,J
通讯作者：
Gatmaitan,J