TISSUE FACTOR IS EXPRESSED ON MONOCYTES DURING SIMULATED EXTRACORPOREAL-CIRCULATION
TISSUE FACTOR IS EXPRESSED ON MONOCYTES DURING SIMULATED EXTRACORPOREAL-CIRCULATION
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DOI：
10.1161/01.res.72.5.1075
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发表时间：
1993-05-01
影响因子：
20.1
通讯作者：
COLMAN, RW
中科院分区：
文献类型：
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作者：
KAPPELMAYER, J;BERNABEI, A;COLMAN, RW
以心肺转流术为例的某些形式的体外循环需要持续的高剂量抗凝以防止血栓栓塞并发症。我们假设，单核细胞可能会受到刺激，表达组织因子（TF）在长期模拟体外循环。采用三种TF特异性单克隆抗体进行流式细胞术和促凝血活性（PCA）功能测定来鉴定TF。通过两项分析，TF在模拟体外循环2 - 6小时之间显著增加。单核细胞上的相对荧光从对照值100增加到来自模拟体外循环的细胞上的313+/-79（p
Certain forms of extracorporeal circulation exemplified by cardiopulmonary bypass require continuous high-dose anticoagulation to prevent thromboembolic complications. We hypothesized that monocytes may be stimulated to express tissue factor (TF) during prolonged simulated extracorporeal circulation. TF was identified both by flow cytometry by using three TF-specific monoclonal antibodies and functional assay of procoagulant activity (PCA). TF significantly increased between 2 and 6 hours of simulated extracorporeal circulation by both analyses. Relative fluorescence on monocytes increased from a control value of 100 to 313+/-79 on cells from the simulated extracorporeal circuit (p