Immunohistochemical determination of rat spinal cord substance P, and antinociceptive effect during development of thiamine deficiency
Immunohistochemical determination of rat spinal cord substance P, and antinociceptive effect during development of thiamine deficiency
复制标题
大鼠脊髓 P 物质的免疫组织化学测定以及硫胺素缺乏期间的镇痛作用
DOI：
10.1016/0006-8993(95)00718-6
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
K. Kisara
中科院分区：
文献类型：
--
作者：
T. Tadano;T. Asao;T. Aizawa;S. Sakurada;Y. Abe;A. Yonezawa;R. Ando;Y. Arai;H. Kinemuchi;K. Kisara
在硫胺素缺乏(TD)喂养30天期间，大鼠对伤害性热刺激的抗伤害效应(痛阈值)与脊髓内P物质(SP)荧光强度的降低成比例增加。仅一次注射盐酸硫胺素(0.5 mg/kg，S.C.)在TD喂养的早期处理日，有效地将镇痛效果逆转到配对喂养的对照水平。而如果硫胺素治疗在较晚的一天进行，则没有发现这种逆转作用。然而，无论哪一天，除杀戮外，TD诱导的各种动物行为均完全消失。这些结果表明，在一定程度的TD发展后，TD引起的行为效应可能是可逆的，但传入神经纤维可能受到不可逆的损害，其机制可能与兴奋性毒素(S)介导的特定脑区损伤(S)类似。上述结果还提示脊髓背侧的SP和兴奋性毒素谷氨酸可能在伤害性热刺激引起的痛觉传递中起重要作用。
During 30 days of thiamine deficiency (TD) feeding, the rat antinociceptive effect (pain threshold) to noxious heat stimulation was significantly increased in proportion to the decrease substance P (SP) fluorescent intensity in the spinal cord. Only a single injection of thiamine HCl (0.5 mg/kg, s.c.) on the early treatment day during TD feeding effectively reversed the analgesic effect to the pair-fed control level. Whereas this reversal effect by thiamine treatment was not found if this treatment was done on the relatively late day. However, either treatment day, except muricide, complete disappearance of various animal behaviours induced by TD was found. These results indicate that, after certain degree of TD development, TD-induced behavioral effects might be reversible, but the afferent nerve fibers might be irreversibly damaged, probably by the similar mechanism as found for an excitotoxin(s) mediated injury in the certain brain region(s). The results also suggest a possibly that SP and an excitotoxin, glutamate, in the dorsal part of the spinal cord greatly contribute to the pain transmission induced by noxious heat stimulation.