On the chromatography mechanism of reversed-phase liquid chromatography
On the chromatography mechanism of reversed-phase liquid chromatography
复制标题
反相液相色谱的色谱机理探讨
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
P. Zhu
中科院分区：
文献类型：
--
作者：
P. Zhu
SummaryAn equation is derived which can describe how the retention of solutes is influenced by the composition of the mobile phase in reversed-phase liquid chromatography, the retention of solutes in alkyl bonded stationary phase regarded as the complexation between solute molecule and the active sites on the surface of the stationary phase. When the stationary phase is not fully saturated by the organic modifier, the activity of the active sites, the activity coefficient of the adsorbed solute as well as the activity coefficient of the solute in the mobile phase depend on the composition of the mobile phase. However, when the stationary phase is fully saturated, the composition of the mobile phase mainly influences the activity coefficient of the solute in the mobile phase. In addition, the selectivity of retention is discussed in terms of the derived equation.
