CIS-ACTING, RECOMBINATION-STIMULATING ACTIVITY IN A FRAGMENT OF THE RIBOSOMAL DNA OF S-CEREVISIAE
CIS-ACTING, RECOMBINATION-STIMULATING ACTIVITY IN A FRAGMENT OF THE RIBOSOMAL DNA OF S-CEREVISIAE
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DOI：
10.1016/0092-8674(84)90016-3
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
ROEDER, GS
中科院分区：
文献类型：
--
作者：
KEIL, RL;ROEDER, GS
可能存在刺激某些多基因家族的几乎相同的重复单元之间重组的特殊机制，以维持其序列同质性。在酵母串联重复的 rRNA 基因中发现了这种重组刺激活性的证据。酵母核糖体 DNA (rDNA) 片段包含 5S 基因、非转录间隔 DNA 和部分 25S 基因，当插入到酵母基因组的新位置时，会引起局部重组刺激。 rDNA 片段刺激染色体间和染色体内有丝分裂重组，但不刺激减数分裂重组。为了刺激有丝分裂重组，该片段必须作用于重组基因的两个拷贝。 rDNA 片段只有在插入靠近重组等位基因的 5S 基因和远离重组等位基因的 25S 基因时才会刺激交换。
Special mechanisms for stimulating recombination among the nearly identical repeat units of certain multigene families may exist in order to maintain their sequence homogeneity. Evidence for such a recombination-stimulating activity was found in the tandemly repeated rRNA genes of yeast. A fragment of the yeast ribosomal DNA (rDNA), containing the 5S gene, nontranscribed spacer DNA and part of the 25S gene, causes a localized stimulation of recombination when inserted at novel locations in the yeast genome. The rDNA fragment stimulates interchromosomal and intrachromosomal mitotic recombination, but not meiotic recombination. To stimulate mitotic recombination, the fragment must act on both copies of the recombining gene. The rDNA fragment stimulates exchange only when inserted with the 5S gene proximal to, and the 25S gene distal to, the recombining alleles.