Polymorphisms in the promoter region of the bovine lactoferrin gene influence milk somatic cell score and milk production traits in Chinese Holstein cows
Polymorphisms in the promoter region of the bovine lactoferrin gene influence milk somatic cell score and milk production traits in Chinese Holstein cows
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牛乳铁蛋白基因启动子区多态性影响中国荷斯坦奶牛乳体细胞评分和产奶性状
DOI：
10.1016/j.rvsc.2015.09.021
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发表时间：
2015-12-01
影响因子：
2.4
通讯作者：
Yang, Zhangping
中科院分区：
文献类型：
--
作者：
Mao, Yongjiang;Zhu, Xiaorui;Yang, Zhangping
乳铁蛋白是一种存在于牛奶中的铁结合蛋白，在预防乳房内感染引起的乳腺炎中起重要作用。本研究随机选取20头中国荷斯坦奶牛，对牛乳铁蛋白基因启动子区进行PCR扩增和序列测定，并对-461 ~-132位点进行SNP发现。在牛乳铁蛋白中鉴定了三个SNP（-270 T > C、-190 G > A和-156 A > G），然后使用Sequenom MassARRAY（Sequenom Inc.，San Diego，CA）的单倍型，并利用SAS软件中的GLM程序中的最小二乘法分析单倍型和单倍型与奶牛体细胞评分（SCS）及生产性状之间的关系。SNPs-270 T > C和-156 A > G的连锁不平衡关系密切（r（2）= 0.76）。SNP - 190 G > A与SCS显著相关，GG基因型个体的SCS显著高于AG和M基因型个体。SNPs-270 T > C和-190 G > A与SCS和乳成分相关。软件Matlnspector显示这些SNPs位于几个潜在的转录因子结合位点，包括NF-κ B p50，KLF 7和SP 1，并可能改变基因表达，但需要进一步的研究来阐明这些SNPs的生物学和实际意义。(C)2015爱思唯尔有限公司版权所有。
Lactoferrin is an iron-binding protein found in cow's milk that plays an important role in preventing mastitis caused by intramammaty infection. In this study, 20 Chinese Holstein cows were selected randomly for PCR amplification and sequencing of the bovine lactoferrin gene promoter region and used for SNP discovery in the region between nucleotide positions -461 to -132. Three SNPs (-270 T > C, -190 G > A and -156 A > G) were identified in bovine lactoferrin, then Chinese Holstein cows (n = 866) were genotyped using Sequenom MassARRAY (Sequenom Inc., San Diego, CA) based on the previous SNP information in this study, and the associations between SNPs or haplotype and milk somatic cell score (SCS) and production traits were analyzed by the least squares method in the GLM procedure of SAS. SNPs -270 T > C and -156 A > G showed close linkage disequilibrium (r(2) = 0.76). The SNP - 190 G > A showed a significant association with SCS, and individuals with genotype GG had higher SCS than genotypes AG and M. Associations were found between the SNPs -270 T > C and -190 G > A with SCS and the milk composition. The software Matlnspector revealed that these SNPs were located within several potential transcription factor binding sites, including NF-kappa B p50, KLF7 and SP1, and may alter gene expression, but further investigation will be required to elucidate the biological and practical relevance of these SNPs. (C) 2015 Elsevier Ltd. All rights reserved.