Cell surface-associated growth inhibitory proteins: Evidence for conservation between mouse and human cell lines
Cell surface-associated growth inhibitory proteins: Evidence for conservation between mouse and human cell lines
复制标题
细胞表面相关生长抑制蛋白：小鼠和人类细胞系之间保守性的证据
DOI：
10.1016/0014-4827(81)90334-7
复制
发表时间：
1981
影响因子：
3.7
通讯作者：
L. Glaser
中科院分区：
文献类型：
--
作者：
M. Lieberman;D. Raben;L. Glaser
正常人成纤维细胞系（IMR 91）表现出密度依赖性生长调节的能力已被检查。该细胞系表现出密度依赖性生长调节，在15%胎牛血清中的饱和密度为2 × 105个细胞/cm 2。从这些细胞的汇合单层制备的膜含有对指数生长的IMR 91和Swiss 3 T3细胞的生长抑制因子。该因子似乎与先前描述的在Swiss 3 T3细胞表面上发现的因子相似[14]。膜对生长中的IMR 91培养物中DNA合成的抑制具有时间和浓度依赖性。高血清可逆转此作用。利用Swiss 3 T3细胞制备的膜进行的专属性实验表明，膜对抑制具有某些种属专属性，但溶解的膜制剂不再表现出这种专属性。这些结果与生长抑制因子及其受体在进化过程中是保守的这一观点是一致的。
The ability of the normal human fibroblast line (IMR91) to exhibit density-dependent regulation of growth has been examined. The line exhibits density-dependent regulation of growth; saturation density in 15% fetal bovine serum is 2 × 105cells/cm2. Membranes prepared from confluent monolayers of these cells contained growth inhibitory factors to both exponentially growing IMR91 and Swiss 3T3 cells. This factor(s) appears to be similar to a previously described factor found on the surface of Swiss 3T3 cells [14]. The inhibition of DNA synthesis in growing IMR91 cultures by membranes was both time- and concentration-dependent. The effect was reversible by high serum. Specificity experiments utilizing membranes prepared from Swiss 3T3 cells indicated some species specificity for inhibition by membranes, but this specificity was no longer exhibited by solubilized membrane preparations. These results are compatible with the suggestion that both the growth inhibitory factors and their receptors are conserved through evolution.