Excess copper promotes catabolic activity of gram-positive bacteria and resistance of gram-negative bacteria but inhibits fungal community in soil
Excess copper promotes catabolic activity of gram-positive bacteria and resistance of gram-negative bacteria but inhibits fungal community in soil
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过量的铜促进革兰氏阳性菌的分解代谢活性和革兰氏阴性菌的抵抗力，但抑制土壤中的真菌群落
DOI：
10.1007/s11356-021-17510-6
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发表时间：
2021-11-18
影响因子：
5.8
通讯作者：
Yang，Yonghua
中科院分区：
文献类型：
--
作者：
Yang，Minkai;Liu，Yehao;Yang，Yonghua
铜杀菌剂的广泛使用对土壤微生物群落产生了显着的非目标效应。然而，记录的效果往往是可变的和矛盾的，这取决于用于评估它们的方法。在这项研究中，我们研究了表层土壤中铜积累对微生物分解代谢活性、活性生物量和组成以及敏感细菌种类的影响。群落水平分解代谢谱（CLCP）显示，正常（50 mg CuSO4kg−1soil）和高剂量（十倍）CuSO4 均显着增加了革兰氏阳性菌的分解代谢多样性，而高剂量增加了革兰氏阴性菌的总体分解代谢活性。磷脂脂肪酸(PLFA)分析表明，高剂量使革兰氏阳性菌生物量降低27%，但对革兰氏阴性菌没有影响。相比之下，正常剂量和高剂量分别使真菌生物量减少了 34% 和 58%。此外，16S rRNA 变性梯度凝胶电泳 (DGGE) 指纹显示，超过三分之二的已识别条带属于革兰氏阴性菌。在施用高剂量CuSO4的土壤中检测到一些耐铜的革兰氏阴性菌属，如放线菌属、假单胞菌属和变形菌属。总之，过量使用 CuSO4 增加了革兰氏阳性菌的分解代谢多样性，并诱导革兰氏阴性菌产生耐药性，而活跃的真菌群落对 CuSO4 表现出剂量依赖性反应，因此可以用作铜污染的敏感指标。
The extensive use of copper fungicides has resulted in significant non-target effects on soil microbial communities. However, the documented effects are often variable and contradictory, depending on the methods used to assess them. In this study, we examined the effects of copper accumulation in surface soils on microbial catabolic activity, active biomass and composition, and sensitive bacterial species. The community-level catabolic profiles (CLCPs) showed that both normal (50 mg CuSO4kg−1soil) and high dosages (tenfold rate) of CuSO4significantly increased the catabolic diversity of gram-positive bacteria, while the high dosage increased the overall catabolic activity of gram-negative bacteria. The phospholipid fatty acid (PLFA) analysis showed that the high dosage reduced the biomass of gram-positive bacteria by 27% but did not affect that of gram-negative bacteria. In comparison, the normal and high dosages decreased the fungal biomass by 34% and 58%, respectively. Furthermore, 16S rRNA-denaturing gradient gel electrophoresis (DGGE) fingerprint revealed that more than two-thirds of identified bands belonged to gram-negative bacteria. Some Cu-resistant gram-negative bacterial genera, such asActinobacterium,Pseudomonas, andProteobacterium, were detected in the soil to which the high dosage of CuSO4had been applied. In conclusion, an excess application of CuSO4increased the catabolic diversity of gram-positive bacteria and induced resistance in gram-negative bacteria, whereas the active fungal community displayed a dosage-dependent response to CuSO4and can thus be used as a sensitive indicator of copper contamination.