Impairment of testicular function in adult male Japanese quail (Coturnix japonica) after a single administration of 3-methyl-4-nitrophenol in diesel exhaust particles
Impairment of testicular function in adult male Japanese quail (Coturnix japonica) after a single administration of 3-methyl-4-nitrophenol in diesel exhaust particles
复制标题
DOI：
10.1677/joe.1.06628
复制
发表时间：
2006-06-01
影响因子：
4
通讯作者：
Taya, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Li, ChunMei;Takahashi, Shinji;Taya, Kazuyoshi
研究了柴油机尾气中的一种成分 3-甲基-4-硝基苯酚 (PNMC) 对成年雄性日本鹌鹑生殖功能的影响。鹌鹑用单一的肌内注射处理。 PNMC剂量（78、103或135 mg/kg体重），并在1、2或4周后收集躯干血液和睾丸。在所有接受 PNMC 治疗的组中均观察到不同程度的睾丸萎缩。睾丸萎缩的鸟类的精子形成、泄殖腔腺面积以及血浆 LH 和睾酮浓度也减少。为了确定 PNMC 对垂体促性腺激素的急性作用，对成年雄性鹌鹑进行单次肌内注射。注射PNMC (25 mg/kg)，并在1、3和6小时测定血浆LH浓度。该剂量显着降低了所有三个时间点的 LH 血浆水平。这些结果表明，PNMC 通过在给药后数小时内减少循环 LH 并随后减少睾酮分泌来作用于下丘脑-垂体轴。此外，为了研究PNMC对鹌鹑睾丸睾丸细胞分泌睾酮的直接影响，将培养的含有Leydig细胞的间质细胞暴露于PNMC（10（-6）、10（-5）或10（-4）M）4、8或24 h。这些数量的 PNMC 以时间和剂量依赖性方式显着减少睾酮的分泌。目前的研究结果还表明 PNMC 对睾丸有直接作用，可减少睾酮分泌。这项研究清楚地表明，PNMC 在中枢和睾丸水平上都会引起生殖毒性，并破坏成年雄性鹌鹑的睾丸功能。
The effects of 3-methyl-4-nitrophenol (PNMC), a component of diesel exhaust, on reproductive function were investigated in adult male Japanese quail. The quail were treated with a single i.m. dose of PNMC (78, 103 or 135 mg/kg body weight), and trunk blood and testes were collected 1, 2 or 4 weeks later. Various levels of testicular atrophy were observed in all groups treated with PNMC. Sperm formation, cloacal gland area, and plasma LH and testosterone concentrations were also reduced in birds with testicular atrophy. To determine the acute effect of PNMC on gonadotrophin from the pituitary, adult male quail were administrated a single i.m. injection of PNMC (25 mg/kg), and plasma concentrations of LH were measured at 1, 3 and 6 h. This dose significantly lowered plasma levels of LH at all three time points. These results suggest that PNMC acts on the hypothalamus-pituitary axis, by reducing circulating LH within a few hours of administration and subsequently reducing testosterone secretion. In addition, in order to investigate the direct effects of PNMC on the secretion of testosterone from testicular cells in quail testes, cultured interstitial cells containing Leydig cells were exposed to PNMC (10(-6), 10(-5) or 10(-4) M) for 4, 8 or 24 h. These quantities of PNMC significantly reduced the secretion of testosterone in a time- and dose-dependent manner. The present findings also suggest a direct effect of PNMC on the testis to reduce testosterone secretion. This study clearly indicates that PNMC induces reproductive toxicity at both the central and testicular levels, and disrupts testicular function in adult male quail.