Development and characterization of microsatellite markers in Brassica rapa ssp. chinensis and transferability among related species.
Development and characterization of microsatellite markers in Brassica rapa ssp. chinensis and transferability among related species.
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DOI：
10.1016/s1671-2927(08)60018-8
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Jin, M. F.
中科院分区：
文献类型：
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作者：
Cui XiuMin, Cui XiuMin;Dong YuXiu, Dong YuXiu;Jin, M. F.
简单序列重复(SSR)或微卫星标记是一种有价值的工具，可用于作图、指纹分析和育种等多种目的。本研究以不结球白菜为材料，利用内部简单序列重复序列(1SSR)-聚合酶链式反应技术开发SSR标记。共获得190个SSR。其中，AG或CT重复频率最高(54.7%)，其次是AC或GT(31.6%)。SSR长度排列的平均数分别为16和10倍。根据测得的SSR序列，设计了143对SSR引物，用来评价它们在油菜属近缘种间的可转移性。平均每个标记产生的等位基因数为2.91个，多态信息含量范围为0~0.863，平均为0.540。16对引物均呈单态现象。CC基因组的可转移率高于BB基因组。BBCC基因组中出现了更多的基因座。这一结果支持了BB基因组与A和C基因组的分化，AA和CC基因组相对接近的假说。该多态引物可用于进一步的进化、指纹分析和品种鉴定。
Simple sequence repeat (SSR) or microsatellite marker is a valuable tool for several purposes, such as mapping, fingerprinting, and breeding. In the present study, an inter-simple sequence repeat (1SSR)-PCR technique was applied for developing SSR markers in non-heading Chinese cabbage (Brassica rapa [B. chinensis]). A total of 190 SSRs were obtained. Among these, AG or CT (54.7%) was the most frequent repeat, followed by AC or GT (31.6%) of the microsatellites. The average number of the SSRs length array was 16 and 10 times, respectively. Based on the determined SSR sequences, 143 SSR primer pairs were designed to evaluate their transferabilities among the related species of Brassica. The number of alleles produced per marker averaged 2.91, and the polymorphism information content (PIC) value ranged from 0 to 0.863 with an average of 0.540. Monomorphism was observed in 16 primer pairs. The transferability percentage in CC genome was higher than in BB genome. More loci occurred in the BBCC genome. This result supported the hypothesis that BB genome was divergent from A and C genomes, and AA and CC genomes were relatively close. The polymorphic primers can be exploited for further evolution, fingerprinting, and variety identification.