Molecular variation and phylogeny of members of the Minimus Group of Anopheles subgenus Cellia (Diptera: Culicidae)
Molecular variation and phylogeny of members of the Minimus Group of Anopheles subgenus Cellia (Diptera: Culicidae)
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DOI：
10.1046/j.1365-3113.2000.00118.x
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发表时间：
2000-04
影响因子：
4.8
通讯作者：
R. G. Sharpe;R. Harbach;R. Butlin
中科院分区：
文献类型：
--
作者：
R. G. Sharpe;R. Harbach;R. Butlin
对按蚊亚属极小群的四个成员的种内和种间分子变异进行了研究：按蚊。乌头，An.瓦鲁纳，安.最小值 A 和 An。报道了这些物种之间在线粒体基因座（细胞色素氧化酶 II）和三个核基因座（rDNA 的 ITS2 和 D3 区域以及鸟苷酸环化酶）处的 DNA 序列差异。数据证实 An 中存在两种神秘物种 A 和 C。 minimus 并为第三个物种的存在提供证据。据估计，微小按蚊 A 和 C 在 0.57-150 万年前就已经分化。讨论了使用鸟苷酸环化酶内含子观察到的差异，鸟苷酸环化酶内含子是冈比亚复合体中已知进化最快的区域，但在 Minimus 群中进化相对较慢。 An 的长期有效种群规模。 minimus A 和 C 估计有数百万，其中物种 A 的大小大约是物种 C 的两倍。这意味着 An。 minimus C 的分布范围比目前认识的要广泛得多，可能对病媒控制具有广泛的影响。在物种 A 或 C 中都没有发现种群结构的证据：内部线粒体单倍型的变异比地方之间的变异更大。重建了Myzomyia系列东方成员的系统发育关系。
Intra‐ and interspecific molecular variation were investigated in four members of the Minimus Group of Anopheles subgenus Cellia: An. aconitus, An. varuna, An. minimus A and An. minimus C. DNA sequence divergence between these species at a mitochondrial locus (cytochrome oxidase II) and at three nuclear loci (ITS2 and D3 regions of rDNA and guanylate cyclase) is reported. The data confirm the presence of two cryptic species, A and C, within An. minimus and provide evidence for the existence of a third species. Anopheles minimus A and C are estimated to have diverged 0.57–1.5 million years ago. The discrepancy observed using the guanylate cyclase intron, which is the fastest evolving region known in the Gambiae Complex but is relatively slowly evolving in the Minimus Group, is discussed. The long‐term effective population sizes of An. minimus A and C are estimated to be in their millions, with that of species A being approximately twice the size of species C. This implies that An. minimus C has a much wider distribution than currently recognized, with possible widespread implications for vector control. No evidence was found for population structuring in either species A or C: there was greater variation of mitochondrial haplotypes within than among localities. The phylogenetic relationships of Oriental members of the Myzomyia Series are reconstructed.