Efficient entry of budded virions of Autographa californica multiple nucleopolyhedrovirus into Spodoptera frugiperda cells is dependent on dynamin, Rab5, and Rab11
Efficient entry of budded virions of Autographa californica multiple nucleopolyhedrovirus into Spodoptera frugiperda cells is dependent on dynamin, Rab5, and Rab11
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苜蓿银纹夜蛾多核多角体病毒的出芽病毒粒子有效进入草地贪夜蛾细胞取决于动力、Rab5 和 Rab11
DOI：
10.1016/j.ibmb.2020.103409
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发表时间：
2020
影响因子：
3.8
通讯作者：
Li Zhaofei
中科院分区：
文献类型：
--
作者：
Yue Qi;Li Jingfeng;Guo Ya;Yan Fanye;Liu Ximeng;Blissard Gary;Li Zhaofei
加州签名多核多角体病毒（AcMNPV）是杆状病毒科甲型杆状病毒属的一种包膜双链DNA病毒。AcMNPV的出芽病毒粒子（BVs）通过网格蛋白介导的内吞作用进入宿主细胞。然而，在进入过程中，AcMNPV bv在细胞内的功能性运输途径尚不清楚。在目前的研究中，我们发现进入bv主要与细胞Rab5和Rab11共定位。显性阴性（DN） Rab5和Rab11的表达或rnai介导的这两种细胞转录物的下调可显著减少bv进入但不从spodoptera frugiperdacells （Sf9）中流出，而Rab4和Rab7的类似处理对病毒感染没有明显影响。结合RNAi敲除dynamin和dyore抑制实验的数据，我们的研究结果支持一个模型，在这个模型中，AcMNPV bv通过网格蛋白介导的内吞作用进入允许的宿主细胞，随后bv主要在早期和成熟的核内体而不是在晚期核内体中去包膜。此外，Rab11抑制研究表明Rab11依赖的再循环内体途径参与了病毒粒子的进入。
Autographa californica multiple nucleopolyhedrovirus (AcMNPV), a member of theAlphabaculovirusgenus of the familyBaculoviridae, is an enveloped double-stranded DNA virus. Budded virions (BVs) of AcMNPV enter host cells via clathrin-mediated endocytosis. However, the route of functional intracellular trafficking of AcMNPV BVs during entry is not well established. In the current study, we found that entering BVs were colocalized mainly with cellular Rab5 and Rab11. Expression of dominant-negative (DN) Rab5 and Rab11 or RNAi-mediated down regulation of these two cellular transcripts significantly reduced BVs entry into but not egress fromSpodoptera frugiperdacells (Sf9), whereas similar treatments for Rab4 and Rab7 had no apparent effect on virus infection. Combined with data from RNAi knockdowns of dynamin, and dynasore inhibition assays, our results support a model in which AcMNPV BVs enter permissive host cells by clathrin-mediated endocytosis, followed by de-envelopment of BVs predominantly within early and maturing endosomes rather than within late endosomes. Additionally, Rab11 suppression studies suggest the Rab11-dependent recycling endosomal pathway is involved in virion entry.