Increased sodium channel immunofluorescence at myelinated and demyelinated sites following an inflammatory and partial axotomy lesion of the rat infraorbital nerve
Increased sodium channel immunofluorescence at myelinated and demyelinated sites following an inflammatory and partial axotomy lesion of the rat infraorbital nerve
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DOI：
10.1016/j.pain.2006.05.028
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发表时间：
2006-09-01
期刊：
影响因子：
7.4
通讯作者：
Levinson, S. Rock
中科院分区：
文献类型：
--
作者：
Henry, Michael A.;Freking, Angelique R.;Levinson, S. Rock
神经损伤后，Chatiriels钠(NaChs)的定位发生变化，这种变化可能有助于疼痛状态的增加。在这里，我们研究了炎性/部分轴突联合损伤后两周大鼠眶下神经(ION)内NaCHs分布的变化，该损伤导致行为对机械刺激的敏感性增加。用识别所有NACH异构体的抗体和识别Ranvier结节的CASPR抗体对实验离子和正常对照离子的切片进行间接免疫荧光双重染色，然后得到共聚焦显微镜Z系列光学切片图像。使用ImageJ(NIH)软件对CASPR和非CASPR相关累积内显示最大NACH强度的像素面积进行量化。分析表明，与正常离子相比，受损离子有更多的分裂结节、半结节和CASPR阴性的“裸露”堆积，典型结节内NACH染色面积显著增加和“裸露”堆积，以及显著堆积的密度和大小增加。这项研究证明了NaChs的戏剧性重新分布和增强的免疫荧光，特别是在病变近端纤维中的肌斜和脱髓鞘部位。本研究中所见的NaChs重塑可能是与损伤后神经兴奋性增加相关的一个重要事件。(C)2006年国际疼痛研究协会。爱思唯尔出版，版权所有。
The localization of sodium chatiriels NaChs) change following nerve lesions and this change may contribute to the development of increased pain states. Here we examine the change in distribution of NaChs within the rat infraorbital nerve (ION) two weeks after a combined inflammatory/partial axotomy lesion that results in behavior showing increased sensitivity to mechanical stimuli. Sections from experimental and normal control IONs were double-stained for indirect immunofluorescence using an antibody that identifies all NaCh isoforms and caspr-antibody to identify nodes of Ranvier, and a confocal microscope z-series of optically sectioned images were then obtained. ImageJ (NIH) software was used to quantify the area of pixels showing maximum NaCh intensity within both caspr and non-caspr associated accumulations. Analysis showed that the lesioned IONs had many more split nodes, heminodes and caspr-negative "naked" accumulations, a significantly increased area of NaCh staining within typical nodes and "naked" accumulations, as well as an increased density and size of significant accumulations when compared to normal IONs. This study demonstrates a dramatic redistribution and increased immunofluorescence of NaChs especially at myclinated and dernyelinated sites in fibers located just proximal to the lesion. The remodeling of NaChs seen in this study may represent an important event associated with the development of increased nerve excitability after lesions. (c) 2006 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.