Molecular phylogeny of Conradina and other scrub mints (Lamiaceae) from the southeastern USA:: Evidence for hybridization in Pleistocene refugia?
Molecular phylogeny of Conradina and other scrub mints (Lamiaceae) from the southeastern USA:: Evidence for hybridization in Pleistocene refugia?
复制标题
DOI：
10.1600/036364406775971688
复制
发表时间：
2006-01-01
影响因子：
1
通讯作者：
Soltis, PS
中科院分区：
文献类型：
--
作者：
Edwards, CE;Soltis, DE;Soltis, PS
Conradina（唇形科）由美国东南部特有的六种异域物种组成，其中四种是联邦濒危或受威胁的物种。一些类群的限制和地位基于形态学理由存在争议，并且澄清这些限制对于设计和实施有效且在财政上负责的保护和管理计划是必要的。本研究的目的是调查康拉迪纳薄荷的单系性及其与美国东南部其他特有薄荷的关系，了解康拉迪纳薄荷的多样化模式，并澄清物种关系。通过对 Conradina 各物种的多个种质（C. verticillata 的单个种质除外）和 Clinopodium、Dicerandra、Piloblephis、Stachydeoma、Monarda、Pycnanthemum 和 Mentha 种的多个个体的 ITS 和质体区域进行测序，推断出分子系统发育。 ITS 序列数据强烈支持 Conradina 的单系性，与形态学证据一致。相反，质体序列数据不支持单系Conradina，并将该属与Clinopodium、Stachydeoma 和Piloblephis 并列。不同属共享相似的质体单倍型，可能是由于共享祖先多态性，或更可能是最近或更新世期间发生的基因渗入。在 Conradina 中，ITS 序列数据不能解决物种关系，而质体序列数据不支持大多数传统上定义的在形态上可区分的 Conradina 物种的单系性。质体数据集中的物种关系也可能因基因渗入或祖先多态性而变得模糊。为了澄清康拉迪纳和美国东南部相关铸币厂的关系，需要来自核标记的更快速演变的序列数据。
Conradina (Lamiaceae) consists of six allopatric species endemic to the southeastern United States, four of which are federally endangered or threatened. The limits and status of several taxa have been contested based on morphological grounds, and clarification of these limits is necessary for the design and implementation of effective and fiscally responsible protection and management plans. The objectives of this study were to investigate the monophyly of Conradina and its relationship to other endemic mints of the southeastern United States, to understand the patterns of diversification in Conradina, and to clarify species relationships. A molecular phylogeny was inferred by sequencing ITS and plastid regions from multiple accessions of each species of Conradina (except for a single accession of C. verticillata) and multiple individuals from species of Clinopodium, Dicerandra, Piloblephis, Stachydeoma, Monarda, Pycnanthemum, and Mentha. ITS sequence data strongly support the monophyly of Conradina, in agreement with evidence from morphology. In contrast, plastid sequence data do not support a monophyletic Conradina and place the genus as paraphyletic to Clinopodium, Stachydeoma, and Piloblephis. Similar plastid haplotypes are shared by different genera, perhaps due to shared ancestral polymorphisms, or more likely, introgression that occurred recently or during the Pleistocene. Within Conradina, ITS sequence data do not resolve species relationships, while plastid sequence data do not support the monophyly of most traditionally defined species of Conradina that are distinguishable morphologically. Species relationships in the plastid data set may also be obscured by introgression or ancestral polymorphism. More rapidly evolving sequence data from nuclear markers will be necessary to clarify relationships in Conradina and related mints from the southeastern United States.