Biological control of the root-knot nematode Meloidogyne incognita by Paecilomyces lilacinus strain 251
Biological control of the root-knot nematode Meloidogyne incognita by Paecilomyces lilacinus strain 251
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DOI：
10.1016/j.biocontrol.2005.12.006
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发表时间：
2006-08-01
影响因子：
4.2
通讯作者：
Sikora, R. A.
中科院分区：
文献类型：
--
作者：
Kiewnick, S.;Sikora, R. A.
对淡紫拟青霉251菌株（PL 251）防治番茄根结线虫病的效果进行了评价。在生长室实验中，种植前土壤处理减少了66%的根擦伤，卵块的数量减少了74%，最终线虫种群在根部的接种对照相比，由71%。当分生孢子应用于土壤时，无论有或没有基于葡萄糖的制剂，建立了显着的剂量-反应关系。商业配制产品的有效浓度（50）（EC 50）值范围为0.097 g-0.08 g/500 cm（3）土壤，相当于参数虫瘿指数和每根最终种群的EC 50分别为1.29 x 10（6）和9.88 x 10（5）菌落形成单位（CFU）/g土壤。对于每个根的卵块数量，EC 50为0.007 g产品或2.64 x 10（5）CFU/g土壤。同样，对于虫瘿指数和每个根的最终种群，不使用制剂施用的分生孢子的EC 50值为0.068 g或0.103 g/500 cm（3）土壤（EC 50为8.10 x 10（5）-1.40 x 10（6）CFU/g土壤）。相比之下，每个根的卵团数的EC 50为0.096 g（EC 50为1.28 x 10（6）CFU/g土壤）。我们证明，一个单一的种植前应用的浓度为1 × 10（6）CFU/克土壤是需要足够的生物控制M。未知的PL 251 (c)2005年爱思唯尔公司All rights reserved.
The fungal biocontrol agent, Paecilomyces lilacinus strain 251 (PL251), was evaluated for its potential to control the root-knot nematode Meloidogyne incognita on tomato. In growth chamber experiments, a pre-planting soil treatment reduced root galling by 66%, number of egg masses by 74% and the final nematode population in the roots by 71% compared to the inoculated control. Significant dose-response relationships were established when conidia were applied to soil either with or without the glucose-based formulation. The effective concentration(50) (EC50) values for the commercially formulated product ranged between 0.097 g and 0.08 g/500 cm(3) soil, equivalent to an EC50 of 1.29 x 10(6) and 9.88 x 10(5) colony forming units (CFU)/g soil for the parameters gall index and final population per root, respectively. For the number of egg masses per root the EC50 was 0.007 g product or 2.64 x 10(5) CFU/g soil. Similarly, EC50 values for conidia applied without formulation were 0.068 g or 0.103 g/500 cm(3) soil (EC50 of 8.10 x 10(5)-1.40 x 10(6) CFU/g soil) for gall index and final population per root. In contrast, the EC50 was 0.096 g (EC50 of 1.28 x 10(6) CFU/g soil) for the number of egg masses per root. We demonstrated that a single pre-plant application at a concentration of 1 x 10(6) CFU/g soil is needed for sufficient biocontrol of M. incognita by PL251. (c) 2005 Elsevier Inc. All rights reserved.