Identification and classification of genes that act antagonistically to let-60 Ras signaling in Caenorhabditis elegans vulval development
Identification and classification of genes that act antagonistically to let-60 Ras signaling in Caenorhabditis elegans vulval development
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DOI：
10.1534/genetics.106.056465
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发表时间：
2006-06-01
期刊：
影响因子：
3.3
通讯作者：
Horvitz, H. Robert
中科院分区：
文献类型：
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作者：
Ceol, Craig J.;Stegmeier, Frank;Horvitz, H. Robert
合成多阴门（synMuv）基因对线虫秀丽隐杆线虫中Ras介导的阴门诱导起负调控作用。synMuv基因分为A、B和C三类，任意两类基因发生突变的双突变体都呈现多阴门表型。B类synMuv基因包括lin - 35，它是视网膜母细胞瘤（Rb）肿瘤抑制基因的同源基因，以及在转录调控中与Rb共同起作用的基因的同源基因。我们采用一种不同于以往synMuv基因筛选的策略来筛选其他synMuv突变。我们所获得的一些突变影响了新的synMuv基因。我们提出了将synMuv突变归入不同基因类别的标准。我们还描述了B类synMuv基因lin - 65的分子特征。
The synthetic multivulva (synMuv) genes negatively regulate Ras-mediated vulval induction in the nematode Caenorhabditis elegans. The synMuv genes define three classes, A, B, and C, such that double mutants carrying mutations in genes of any two classes are multivulva. The class B synMuv genes include lin-35, a homolog of the retinoblastoma (Rb) tumor suppressor gene, as well as homologs of genes that function with Rb in transcriptional regulation. We screened for additional synMuv mutations using a strategy different from that of previous synMuv genetic screens. Some of the mutations we recovered affect new synMuv genes. We present criteria for assigning synMuv mutations into different genetic classes. We also describe the molecular characterization of the class B synMuv gene lin-65.