Mass spectrometric characterisation of avenanthramides and enhancing their production by germination of oat (Avena sativa)
Mass spectrometric characterisation of avenanthramides and enhancing their production by germination of oat (Avena sativa)
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DOI：
10.1016/j.foodchem.2018.11.013
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发表时间：
2019-03-30
期刊：
影响因子：
8.8
通讯作者：
Vincken, Jean-Paul
中科院分区：
文献类型：
--
作者：
de Bruijn, Wouter J. C.;van Dinteren, Sarah;Vincken, Jean-Paul
燕麦蒽酰胺是一种酰胺，含有苯基烯酸（PA）和邻氨基苯甲酸（AA）亚基，是燕麦的次级代谢产物。燕麦种子发芽，提取，和avenanthramides分析的超高效液相色谱与离子阱和高分辨率ESI-MS相结合。典型的碎片化途径与相应的诊断片段属于PA和AA亚基进行了鉴定和总结的决策指南。基于这些发现，在燕麦种子（ling）提取物中注释了28种独特的燕麦生物碱，包括新的燕麦生物碱6 f（具有4/5-甲氧基AA亚基）。燕麦生物碱含量从种子到幼苗增加了25倍。燕麦生物碱2 p、2c和2f是燕麦生物碱的主要成分，占总含量的20%以下。因此，未来的定量分析应包括更广泛的燕麦生物碱，以避免低估总燕麦生物碱含量。
Avenanthramides are amides, with a phenylalkenoic acid (PA) and an anthranilic acid (AA) subunit, which are secondary metabolites of oat. Oat seeds were germinated, extracted, and the avenanthramides analysed by a combination of UHPLC with ion trap and high resolution ESI-MS. Typical fragmentation pathways with corresponding diagnostic fragments belonging to the PA and AA subunits were identified and summarised in a decision guideline. Based on these findings 28 unique avenanthramides were annotated in the oat seed(ling) extracts, including the new avenanthramide 6f (with a 4/5-methoxy AA subunit). Avenanthramide content increased by 25 times from seed to seedling. Avenanthramides 2p, 2c, and 2f, which are commonly described as the major avenanthramides, represented less than 20% of the total content in the seedlings. Future quantitative analyses should, therefore, include a wider range of avenanthramides to avoid underestimation of the total avenanthramide content.