A new simple and sensitive fluorometric method for the determination of hydroxyl radical and its application.
A new simple and sensitive fluorometric method for the determination of hydroxyl radical and its application.
复制标题
DOI：
10.1016/s0039-9140(02)00370-3
复制
发表时间：
2002-10
期刊：
影响因子：
6.1
通讯作者：
Chao Tai;X. Gu;H. Zou;Qihua Guo
中科院分区：
文献类型：
--
作者：
Chao Tai;X. Gu;H. Zou;Qihua Guo
本文介绍了一种简便、灵敏的羟基自由基荧光检测方法。该方法利用羟基与二甲基亚砜(DMSO)的反应生成定量的甲醛，然后在pH值为4.5的条件下与氨和1，3-环己二酮(CHD)反应。该产品产生了特征荧光，其激发和发射波长分别为400.4和452.3 nm。通过测定荧光强度，可以对羟基自由基进行定量分析。用建立的方法研究了某些氨基酸和黄酮类化合物对羟基自由基的清除能力。
A simple and sensitive fluorometric method to detect the hydroxyl radical is described. This method employs the reaction between OH and dimethyl sulfoxide (DMSO) to generate quantitatively formaldehyde, which then reacts with ammonia and 1,3-cyclohexanedione (CHD) at pH 4.5. The product produces the characteristic fluorescence with its excitation and emission wavelength at 400.4 and 452.3 nm, respectively. The quantitative analysis of hydroxyl radical can be done through the determination of the fluorescence intensity. By the developed method, the scavenging abilities of some amino acids and flavonoids on hydroxyl radical are studied.