Are some putative glycogen accumulating organisms (GAO) in anaerobic : aerobic activated sludge systems members of the alpha-Proteobacteria?
Are some putative glycogen accumulating organisms (GAO) in anaerobic : aerobic activated sludge systems members of the alpha-Proteobacteria?
复制标题
厌氧：好氧活性污泥系统中的一些假定的糖原积累生物（GAO）是否属于α-变形菌门？
作者：
M. Beer;Y. Kong;R. Seviour
设计用于微生物除磷的活性污泥厂通常不可靠。一种观点认为，在这些过程的厌氧区，聚磷酸盐积累生物（PAO）与另一组细菌（糖原积累生物（GAO））竞争底物。本研究采用荧光原位杂交（FISH）和变性梯度凝胶电泳（DGGE）分析了实验室规模的厌氧：好氧序批式反应器的社区。在α-变形菌门中的鞘氨醇单胞菌属的成员大量存在于生物质具有高糖原含量的除磷能力差的社区中。它们厌氧储存聚-β-羟基链烷酸酯而非需氧储存聚-β-羟基链烷酸酯并且不需氧积累聚磷酸盐的能力与这些生物体在那里表现为GAO一致。没有证据表明，支持一个重要的作用，γ-变形菌作为可能的GAO在这些社区，虽然这些细菌种群已被认为是在其他研究中作为可能的竞争对手的PAO。
Activated sludge plants designed to remove phosphorus microbiologically often perform unreliably. One suggestion is that the polyphosphate-accumulating organisms (PAO) are out-competed for substrates by another group of bacteria, the glycogen-accumulating organisms (GAO) in the anaerobic zones of these processes. This study used fluorescence in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) to analyse the communities from laboratory-scale anaerobic : aerobic sequencing batch reactors. Members of the genus Sphingomonas in the alpha-Proteobacteria were present in large numbers in communities with poor phosphorus removal capacity where the biomass had a high glycogen content. Their ability to store poly-beta-hydroxyalkanoates anaerobically, but not aerobically, and not accumulate polyphosphate aerobically is consistent with these organisms behaving as GAO there. No evidence was found to support an important role for the gamma-Proteobacteria as possible GAO in these communities, although these bacterial populations have been considered in other studies to act as possible competitors for the PAO.