MECHANISM FOR NONSPECIFIC IMMUNITY TO LISTERIA-MONOCYTOGENES IN RATS MEDIATED BY PLATELETS AND THE CLOTTING SYSTEM
MECHANISM FOR NONSPECIFIC IMMUNITY TO LISTERIA-MONOCYTOGENES IN RATS MEDIATED BY PLATELETS AND THE CLOTTING SYSTEM
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DOI：
10.1128/iai.33.2.477-481.1981
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
NELSON, DS
中科院分区：
文献类型：
--
作者：
DAVIES, WA;ACKERMAN, VP;NELSON, DS
A proposed mechanism for nonspecific immunity to L. monocytogenes in rats based on the existence of an activatable lysin is described. Using a DNA release assay, lysin activity was found in serum made from whole blood but not in serum made from platelet-free plasma serum. Washed platelets and platelet lysates exhibited only partial activity as compared with that in serum. This activity as amplified by the addition of platelet-free plasma serum. The activity of the lysin was unaffected by heparin, dialysis, a serine esterase inhibitor or heating to 56.degree. C for 30 min. Effective inhibitors were EDTA and stronger heating (to 65.degree. C). Listeria organisms were found to reduce the recalcified clotting time of platelet-rich plasma in a dose-dependent fashion, indicating that the organisms can exhibit procoagulant activity. The susceptibility of rats to Listeria infection was enhanced by anticoagulant treatment. Rats were infected with Listeria organisms with and without administration of heparin. Heparin-treated rats developed bacteremia; some died. None of the control rats developed bacteremia or died. Natural immunity to Listeria infection is apparently partly due to a platelet-dependent lysin which is activated during clotting and is, in turn, promoted by the Listeria organisms themselves.
