Microfluidic systems for single DNA dynamics.
Microfluidic systems for single DNA dynamics.
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DOI：
10.1039/c2sm26036k
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发表时间：
2012-01-01
期刊：
影响因子：
3.4
通讯作者：
Schroeder CM
中科院分区：
文献类型：
--
作者：
Mai DJ;Brockman C;Schroeder CM
微流体技术的最新进展使得使用单个DNA链进行分子水平的聚合物动力学研究成为可能。基于荧光显微镜的单聚合物研究允许直接观察非平衡聚合物构象和动力学现象，如扩散，松弛和分子拉伸流动途径。微流体装置已经能够精确控制模型流场，以研究软材料的非平衡动力学，装置几何形状包括弯曲通道、交叉槽和微加工障碍物和结构。这篇综述探讨了最近的微流体系统，已经推进了单聚合物动力学的研究，同时确定在该领域的新方向，将进一步阐明聚合物的微观结构和体流变性能之间的关系。
Recent advances in microfluidics have enabled the molecular-level study of polymer dynamics using single DNA chains. Single polymer studies based on fluorescence microscopy allow for the direct observation of non-equilibrium polymer conformations and dynamical phenomena such as diffusion, relaxation, and molecular stretching pathways in flow. Microfluidic devices have enabled the precise control of model flow fields to study the non-equilibrium dynamics of soft materials, with device geometries including curved channels, cross-slots, and microfabricated obstacles and structures. This review explores recent microfluidic systems that have advanced the study of single polymer dynamics, while identifying new directions in the field that will further elucidate the relationship between polymer microstructure and bulk rheological properties.