Transforming growth factor-α expression in benign and malignant human prostatic disease
Transforming growth factor-α expression in benign and malignant human prostatic disease
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转化生长因子-α在良性和恶性人类前列腺疾病中的表达
DOI：
10.1177/172460089200700104
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Leakey Re
中科院分区：
文献类型：
--
作者：
S. Lloyd;Brown Il;Leakey Re
对前列腺疾病的生物学变量的研究不仅可以防止预后良好的患者被过度治疗，而且可以更好地选择合适的治疗方法，并且可以确定新疗法的潜在靶点。本研究利用放射免疫分析和免疫组织化学研究良性和恶性前列腺活检中生长因子转化生长因子-α (TGFα) 的表达，考虑其在恶性上皮转化中的作用并作为预后指标。由于前列腺组织的异质性，生化方法不如更具选择性的免疫组织化学方法令人满意。使用放射免疫测定法，71% 的良性活检组织（范围 0-18.62ng/mg DNA）和 69% 的恶性活检组织（范围 0-11.1ng/mg DNA）具有可检测的 TGFα 水平。 TGFαL 的免疫组织化学染色鉴定出 15% 的良性活检组织（27 个活检组织中的 4 个）和 53% 的恶性活检组织（34 个活检组织中的 18 个）中有表达。在癌前病变和基质成分中也发现了阳性染色，因此暗示该因子在自分泌/旁分泌生长和/或恶性转化中的作用。 TGFα 免疫染色可以增强对癌前病变和恶性腺体小病灶的检测，否则使用标准组织病理学技术很难识别这些病灶。
Investigation of biological variables in prostatic disease may not only prevent patients with a good prognosis being overtreated, but allow better selection of appropriate therapy, and may identify potential targets for novel therapies. This study investigates the growth factor transforming growth factor-α (TGFα) expression in benign and malignant prostatic biopsies using both radioimmunoassay and immunohistochemistry, considering its role in malignant epithelial transformation and as a prognostic indicator. Biochemical methods were less satisfactory than the more selective immunohistochemical methods, due to the heterogeneity of prostatic tissue. Seventy-one percent of benign biopsies (range 0-18.62ng/mg DNA) and 69% of malignant biopsies (range 0-11.1ng/mg DNA) had detectable levels of TGFα using radioimmunoassay. Immunohistochemical staining for TGFaL identified expression in 15% of benign (4 out of 27) and 53% malignant biopsies (18 out of 34). Positive staining was also identified in premalignant lesions and within stromal elements, thus implying the factor's role in autocrine/paracrine growth and/or malignant transformation. Immunostaining for TGFα may enhance detection of premalignant lesions and small foci of malignant glands which are otherwise difficult to identify using standard histopathological techniques.