Transcriptome Analysis Revealed the Embryo-Induced Gene Expression Patterns in the Endometrium from Meishan and Yorkshire Pigs.
Transcriptome Analysis Revealed the Embryo-Induced Gene Expression Patterns in the Endometrium from Meishan and Yorkshire Pigs.
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转录组分析揭示了梅山猪和约克夏猪子宫内膜中胚胎诱导的基因表达模式
DOI：
10.3390/ijms160922692
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发表时间：
2015-09-18
影响因子：
5.6
通讯作者：
Yu M
中科院分区：
文献类型：
--
作者：
Huang J;Liu R;Su L;Xiao Q;Yu M
分别研究了妊娠早期（妊娠第15天）和妊娠中期（妊娠第26天和50天）梅山和约克夏来源的子宫内膜中的表达模式。总共，在三个妊娠阶段，分别鉴定出 689 个和 1649 个注释基因在眉山子宫内膜和约克郡子宫内膜中差异表达。层次聚类分析发现，注释的差异表达基因（DEG）中，73个DEG是梅山子宫内膜特有的，536个DEG是约克郡子宫内膜特有的，228个DEG是梅山和约克郡子宫内膜常见的。随后，根据时间表达模式的相似性，将三种表达模式中的每一种的DEG分为四个不同的类别。通过定量 RT-PCR 验证了微阵列分析确定的表达模式。功能富集分析显示，常见的DEG在类固醇代谢过程和视黄酸受体信号调节途径中富集。梅山子宫内膜中这些独特的DEG参与细胞周期和粘附连接。约克郡子宫内膜特有的 DEG 与 Rho 蛋白信号转导、母体胎盘发育和细胞增殖的调节有关。本研究分别揭示了梅山猪和约克夏猪与子宫内膜重塑相关的不同基因表达模式或途径。梅山或约克郡子宫内膜中的这些独特的DEG可能导致这两个猪品种子宫内膜环境的差异。
The expression patterns in Meishan- and Yorkshire-derived endometrium during early (gestational day 15) and mid-gestation (gestational days 26 and 50) were investigated, respectively. Totally, 689 and 1649 annotated genes were identified to be differentially expressed in Meishan and Yorkshire endometrium during the three gestational stages, respectively. Hierarchical clustering analysis identified that, of the annotated differentially expressed genes (DEGs), 73 DEGs were unique to Meishan endometrium, 536 DEGs were unique to Yorkshire endometrium, and 228 DEGs were common in Meishan and Yorkshire endometriums. Subsequently, DEGs in each of the three types of expression patterns were grouped into four distinct categories according to the similarities in their temporal expression patterns. The expression patterns identified from the microarray analysis were validated by quantitative RT-PCR. The functional enrichment analysis revealed that the common DEGs were enriched in pathways of steroid metabolic process and regulation of retinoic acid receptor signaling. These unique DEGs in Meishan endometrium were involved in cell cycle and adherens junction. The DEGs unique to Yorkshire endometrium were associated with regulation of Rho protein signal transduction, maternal placenta development and cell proliferation. This study revealed the different gene expression patterns or pathways related to the endometrium remodeling in Meishan and Yorkshire pigs, respectively. These unique DEGs in either Meishan or Yorkshire endometriums may contribute to the divergence of the endometrium environment in the two pig breeds.