The dyf-3 gene encodes a novel protein required for sensory cilium formation in Caenorhabditis elegans
The dyf-3 gene encodes a novel protein required for sensory cilium formation in Caenorhabditis elegans
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DOI：
10.1016/j.jmb.2004.12.005
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发表时间：
2005-02-25
影响因子：
5.6
通讯作者：
Koga, M
中科院分区：
文献类型：
--
作者：
Murayama, T;Toh, Y;Koga, M
动物的纤毛神经元对于接受环境刺激很重要。为了了解秀丽隐杆线虫纤毛形态发生的机制，我们分析了 dyf-3 突变体，这些突变体在荧光染料的摄取方面有缺陷并且感觉纤毛结构异常。 dyf-3 突变体的感觉神经元中绿色荧光蛋白的表达表明，该突变体的纤毛发育不良，并且某些感觉神经元的后部投射异常。 dyf-3 基因编码三种具有不同 N 末端的蛋白质。最大的 DYF-3 蛋白有 404 个氨基酸残基，与预测的功能未知的人类蛋白的氨基酸残基有 38% 相同。在 26 个化学感应神经元中检测到功能性 dyf-3::gfp 融合基因的表达，包括 6 个 IL2 神经元、8 对两栖神经元（ASE、ADF、ASG、ASH、ASI、ASJ、ASK 和 ADL）和两对噬粒神经元（PHA 和 PHB）。 dyf-3 cDNA 在 dyf-3 动物的特定神经元中的表达表明 dyf-3 细胞自主地作用于荧光染料摄取。 daf-19 突变体中 dyf-3::gfp 表达的减少表明 dyf-3 表达受 DAF-19 转录因子调节，并且 DYF-3 可能参与鞭毛内运输系统。 (C) 2004 Elsevier Ltd. 保留所有权利。
Ciliated neurons in animals are important for the reception of environmental stimuli. To understand the mechanism of cilium morphogenesis in Caenorhabditis elegans, we analyzed dyf-3 mutants that are defective in uptake of a fluorescent dye and abnormal in sensory cilium structure. Expression of green fluorescent protein in sensory neurons of a dyf-3 mutant revealed that the mutant has stunted cilia and abnormal posterior projections in some sensory neurons. The dyf-3 gene encodes three proteins with different N-terminals. The largest DYF-3 protein has 404 amino acid residues that are 38% identical with those of a predicted human protein of unknown function. Expression of a functional dyf-3::gfp fusion gene is detected in 26 chemosensory neurons, including six IL2 neurons, eight pairs of amphid neurons (ASE, ADF, ASG, ASH, ASI, ASJ, ASK and ADL) and two pairs of phasmid neurons (PHA and PHB). Expression of a dyf-3 cDNA in specific neurons of dyf-3 animals indicated that dyf-3 acts cell-autonomously for fluorescent dye uptake. Reduction of dyf-3::gfp expression in a daf-19 mutant suggests that dyf-3 expression is regulated by DAF-19 transcription factor, and DYF-3 may be involved in the intraflagellar transport system. (C) 2004 Elsevier Ltd. All rights reserved.