Effect of Fluoride in Drinking Water on Fecal Microbial Community in Rats
Effect of Fluoride in Drinking Water on Fecal Microbial Community in Rats
复制标题
饮用水中的氟化物对大鼠粪便微生物群落的影响。
DOI：
10.1007/s12011-021-02617-1
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发表时间：
2021-02-12
影响因子：
3.9
通讯作者：
Pei，Junrui
中科院分区：
文献类型：
--
作者：
Zhong，Nan;Ma，Yongzheng;Pei，Junrui
肠道营养与氟中毒的发生发展密切相关。肠道微生物在肠道营养中起着重要作用。然而，氟对肠道微生物的影响仍不完全清楚。本研究旨在评价氟对粪便微生物的剂量效应以及氟中毒与粪便微生物的关系。结果表明，氟对粪便微生物区系多样性的影响不明显，但丰富度估计值(ACE和CHO)随水氟浓度的增加先增大后减小。在属水平上，150 mg/L氟显著降低了玫瑰芽孢杆菌和严格感觉梭菌的丰度，100 mg/L和150 mg/L氟显著增加了未分类瘤胃球菌和未分类蛭弧菌的丰度。相关分析表明，氟暴露与玫瑰红假单胞菌和尿路杆菌呈负相关，与细小杆菌、未分类反刍球菌科和未分类蛭弧菌呈正相关。氟斑牙与感觉梭状芽胞杆菌、玫瑰红假单胞菌、透热杆菌和巴氏杆菌呈负相关，与细小杆菌、未归类反刍球菌科和未归类痘弧菌呈正相关。综上所述，本研究首次报道饮用水中氟对大鼠粪便微生物区系具有显著的双相效应，一些细菌与氟暴露和氟斑牙密切相关。这些结果表明肠道微生物区系可能在氟中毒中起重要作用，一些细菌可能被开发为氟中毒的生物标志物。
Intestinal nutrition has a close association with the onset and development of fluorosis. Intestinal microbes play a major role in intestinal nutrition. However, the effect of fluoride on intestinal microbes is still not fully understood. This study aimed to evaluate the dose-response of fluoride on fecal microbes as well as the link between fluorosis and fecal microbes. The results showed that fluoride did not significantly alter the diversity of fecal microbiota, but richness estimators (ACE and Chao) increased first, and then decreased with the increase of water fluoride. At the genus level, 150 mg/L fluoride significantly reduced the abundances ofRoseburiaandClostridium sensu stricto, and 100 mg/L and 150 mg/L fluoride obviously increased the abundances ofUnclassified RuminococcaceaesandUnclassified Bdellovibrionales, respectively. The correlation analysis showed fluoride exposure had a negative association withRoseburiaandTuricibacterand was positively associated withPelagibacterium,Unclassified Ruminococcaceae, andUnclassified Bdellovibrionales. Dental fluorosis was negatively associated withClostridium sensu stricto,Roseburia,Turicibacter, andPaenalcaligenesand had a positive association withPelagibacterium,Unclassified Ruminococcaceae, andUnclassified Bdellovibrionales. In conclusion, this study firstly reports fluoride in drinking water has a remarkable biphasic effect on fecal microbiota in rats, and some bacteria are significantly associated with fluoride exposure and dental fluorosis. These results indicate the gut microbiota may play an important role in fluorosis, and some bacteria are likely to be developed as biomarkers for fluorosis.