Bergenin, Acting as an Agonist of PPARγ, Ameliorates Experimental Colitis in Mice through Improving Expression of SIRT1, and Therefore Inhibiting NF-κB-Mediated Macrophage Activation.
Bergenin, Acting as an Agonist of PPARγ, Ameliorates Experimental Colitis in Mice through Improving Expression of SIRT1, and Therefore Inhibiting NF-κB-Mediated Macrophage Activation.
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岩白菜素作为PPARγ激动剂，通过改善SIRT 1表达从而抑制NF-κ B介导的巨噬细胞活化来改善小鼠实验性结肠炎。
DOI：
10.3389/fphar.2017.00981
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wei ZF
中科院分区：
文献类型：
--
作者：
Wang K;Li YF;Lv Q;Li XM;Dai Y;Wei ZF
Bergenin, isolated from the herb of Saxifraga stolonifera Curt. (Hu-Er-Cao), has anti-inflammatory, antitussive and wound healing activities. The aim of the present study was to identify the effect of bergenin on experimental colitis, and explored the related mechanisms. Our results showed that oral administration of bergenin remarkably alleviated disease symptoms of mice with dextran sulfate sodium (DSS)-induced colitis, evidenced by reduced DAI scores, shortening of colon length, MPO activity and pathologic abnormalities in colons. Bergenin obviously inhibited the mRNA and protein expressions of IL-6 and TNF-α in colon tissues, but not that of mucosal barrier-associated proteins occludin, E-cadherin and MUC-2. In vitro, bergenin significantly inhibited the expressions of IL-6 and TNF-α as well as nuclear translocation and DNA binding activity of NF-κB-p65 in lipopolysaccharide (LPS)-stimulated peritoneal macrophages and RAW264.7 cells, which was almost reversed by addition of PPARγ antagonist GW9662 and siPPARγ. Subsequently, bergenin was identified as a PPARγ agonist. It could enter into macrophages, bind with PPARγ, promote nuclear translocation and transcriptional activity of PPARγ, and increase mRNA expressions of CD36, LPL and ap2. In addition, bergenin significantly up-regulated expression of SIRT1, inhibited acetylation of NF-κB-p65 and increased association NF-κB-p65 and IκBα. Finally, the correlation between activation of PPARγ and attenuation of colitis, inhibition of IL-6 and TNF-α expressions, NF-κB-p65 acetylation and nuclear translocation, and up-regulation of SIRT1 expression by bergenin was validated in mice with DSS-induced colitis and/or LPS-stimulated macrophages. In summary, bergenin could ameliorate colitis in mice through inhibiting the activation of macrophages via regulating PPARγ/SIRT1/NF-κB-p65 pathway. The findings can provide evidence for the further development of bergenin as an anti-UC drug, and offer a paradigm for the recognization of anti-UC mechanisms of compound with similar structure occurring in traditional Chinese medicines.
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PPAR-γ 活性调节对非酒精性脂肪肝发展中库普弗细胞 M1/M2 极化的影响
影响因子：
4.6
作者：
Luo W;Xu Q;Wang Q;Wu H;Hua J
通讯作者：
Hua J
影响因子：
9
作者：
Hwang JS;Lee WJ;Kang ES;Ham SA;Yoo T;Paek KS;Lim DS;Do JT;Seo HG
通讯作者：
Seo HG
DOI：
10.4049/jimmunol.181.1.235
发表时间：
2008-07-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Bonfield TL;Thomassen MJ;Farver CF;Abraham S;Koloze MT;Zhang X;Mosser DM;Culver DA
通讯作者：
Culver DA
DOI：
10.1073/pnas.1203218109
发表时间：
2012-08-21
影响因子：
11.1
作者：
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通讯作者：
Kim, Jae-Woo
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影响因子：
4.2
作者：
Liu, Zun-Jing;Liu, Hong-Qiang;Wang, Yu
通讯作者：
Wang, Yu