Biomimetic Collagen-Hydroxyapatite Composite Fabricated via a Novel Perfusion-Flow Mineralization Technique
Biomimetic Collagen-Hydroxyapatite Composite Fabricated via a Novel Perfusion-Flow Mineralization Technique
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DOI：
10.1089/ten.tec.2012.0452
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发表时间：
2013-07-01
影响因子：
3
通讯作者：
Ling, Jian
中科院分区：
文献类型：
--
作者：
Antebi, Ben;Cheng, Xingguo;Ling, Jian
骨组织工程中常用的三维支架材料是矿化胶原-羟基磷灰石（Col/HA）复合材料。传统的矿化技术是用矿物质涂覆胶原支架表面或简单地将胶原和矿物质纳米颗粒混合在一起。这些传统的体外胶原矿化方法与体内骨形成过程不同，通常导致支架不适合骨组织工程。在这项研究中，一个独特的灌注流（即，动态）结合先前描述的聚合物诱导的液体-前体（PILP）方法来制造多孔Col/HA复合材料。动态流动模拟含有矿化离子的生理细胞外液流动，而PILP方法促进HA晶体在胶原纤维内的沉积（即，纤维内矿化）。通过利用动态PILP技术来模拟体内骨形成过程，所得Col/HA复合材料具有类似于人骨小梁的结构和组成。动态和静态矿化方法的比较表明，新的动态技术有利于更有效和均匀的矿物沉积在整个Col/HA复合材料。动态原纤维内矿化方法产生刚性Col/HA复合材料，具有优异的表面性能，有利于细胞附着和生长。在Col/HA复合材料上培养的人间充质干细胞迅速重塑支架，并产生具有广泛的细胞来源的细胞外基质网络的结构。
Prevalent three-dimensional scaffolds for bone tissue engineering are mineralized collagen-hydroxyapatite (Col/HA) composites. Conventional mineralization techniques are either to coat collagen scaffold surfaces with minerals or to simply mix collagen and mineral nanoparticles together. These conventional in vitro collagen mineralization methods are different from the in vivo bone formation process and often result in scaffolds that are not suitable for bone tissue engineering. In this study, a unique perfusion-flow (i.e., dynamic) in conjunction with a previously described polymer-induced liquid-precursor (PILP) method was used to fabricate a porous Col/HA composite. The dynamic flow emulated the physiological extracellular fluid flow containing the mineralization ions, while the PILP method facilitated the deposition of the HA crystals within the collagen fibrils (i.e., intrafibrillar mineralization). By utilizing a dynamic PILP technique to mimic the in vivo bone formation process, the resultant Col/HA composite has a similar structure and compositions like human trabecular bone. A comparison of the dynamic and static mineralization methods revealed that the novel dynamic technique facilitates more efficient and homogenous mineral deposition throughout the Col/HA composite. The dynamic intrafibrillar mineralization method generated stiff Col/HA composites with excellent surface property for cell attachment and growth. The human mesenchymal stem cells cultured on the Col/HA composites quickly remodeled the scaffolds and resulted in constructs with an extensive cell-derived extracellular matrix network.