Polyamines reduces lipid peroxidation induced by different pro-oxidant agents
Polyamines reduces lipid peroxidation induced by different pro-oxidant agents
复制标题
DOI：
10.1016/j.brainres.2004.02.036
复制
发表时间：
2004-05-22
期刊：
影响因子：
2.9
通讯作者：
Rocha, JBT
中科院分区：
文献类型：
--
作者：
Bellé, NAV;Dalmolin, GD;Rocha, JBT
在其他功能中，多胺被认为是自由基清除剂和抗氧化剂。喹啉酸(QA)、硝普钠(SNP)和铁(Fe+2)可刺激自由基的产生和脂质过氧化。在本研究中，我们研究了多胺、精胺和亚精胺对QA诱导的硫代巴比妥酸反应物种(TBARS)产生的自由基和/或醛的清除作用。SNP、Fe+2/EDTA体系和大鼠脑内游离Fe2+。精胺和亚精胺抑制QA诱导的TBARS的产生，但精胺是一种比亚精胺更好的抗氧化物质。精胺也抑制SNP-、Fe+2/EDTA-和游离Fe2+诱导的TBARS的产生，但作用不大。反过来，亚精胺也谨慎地抑制SNP-、Fe+2/EDTA-和游离Fe2+诱导的TBARS的产生。在MK-801存在下，QA诱导的TBARS的产生被多胺显著抑制。此外，阿卡因不影响多胺的还原作用。目前的研究结果表明，多胺的作用与激活NMDA受体无关，而与其抗氧化和清除自由基的特性有关。(C)2004爱思唯尔B.V.保留所有权利。
Polyamines, among other functions, are considered to act as a free radical scavenger and antioxidant. The quinolinic acid (QA), sodium nitroprusside (SNP) and iron (Fe+2) stimulate production of free radicals and lipid peroxidation. In the present study, we investigated the free radical and/or aldehyde scavenger effects of polyamines spermine and spermidine on thiobarbituric acid reactive species (TBARS) production induced by QA. SNP, Fe+2/EDTA system and free Fe2+ in rat brain. Spermine and spermidine inhibited QA-induced TBARS production: however spermine was a better antioxidant than spermidine. Spermine also inhibited SNP-, Fe+2/EDTA- and free Fe2+-induced TBARS production, but had a modest effect. Spermidine, in turn, also discreetly inhibited SNP-, Fe+2/EDTA- and free Fe2+-induced TBARS production. In the presence of MK-801, QA-induced TBARS production was considerably more inhibited by polyamines. In addition, arcaine does not affect the reducer effect of polyamines. The present findings suggest that the observed effects of polyamines are not related to the activation of NMDA receptor but with their antioxidant and free radical scavenger properties. (C) 2004 Elsevier B.V. All rights reserved.