The study of continuous membrane crystallization on lysozyme
The study of continuous membrane crystallization on lysozyme
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溶菌酶连续膜结晶的研究
DOI：
10.1016/j.desal.2007.01.018
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发表时间：
2008-01
期刊：
影响因子：
9.9
通讯作者：
Zhang, Peng
中科院分区：
文献类型：
--
作者：
Zhang, Xinmiao;Wang, Yanhui;Wei, Kegui;Ma, Runyu;Zhang, Peng
膜结晶作为一种新的分离工艺是近几年发展起来的。新的结晶技术声称能够在合适的操作条件下产生完美的晶体。在本研究中，讨论了溶菌酶的膜结晶。研究了各种膜结晶条件对溶菌酶晶习和质量的影响，这些条件包括蛋白质浓度、沉淀剂浓度、结晶液流速、反萃液流速和浓度、结晶液pH和离子强度。还研究了结晶溶液浓度随时间和诱导时间的变化。此外，优化了结晶条件，并获得了所得晶体的红外光谱。获得了完美的溶菌酶晶体。根据蛋白质浓度、pH 值和所用的沉淀盐，获得了两种不同的晶体形式。与传统的结晶方法相比，膜结晶速度更快，初始蛋白浓度更慢。此外，膜结晶可以更好地控制结晶过程，得到更好的晶体。
Membrane crystallization, as a new separation process, was developed in the past few years. The new crystallization technique claims to be capable of producing perfect crystals under suitable operating conditions. In this study, membrane crystallization on lysozyme was discussed in this work. The influence of various membrane crystallization conditions on the crystal habit and quality of lysozyme was studied, and these conditions include protein concentration, precipitant concentration, the flow velocity of crystallization solutions, the flow velocity and concentration of stripping solutions, the pH and also ion strength of crystallization solutions. The changes of crystallization solutions concentration with time and the induction time were also studied. Furthermore, crystallization conditions were optimized, and the IR spectra from crystals obtained were made. Perfect lysozyme crystals were obtained. Two different crystal forms have been obtained, depending upon protein concentration, pH and precipitating salt employed. Compared with the traditional crystallization methods, membrane crystallization is more rapid and with slower initial protein concentration. In addition, membrane crystallization can control the crystallization process better, and better crystals were obtained.
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