Study on the Sliding Friction of Endothelial Cells Cultured on Hydrogel and the Role of Glycocalyx on Friction Reduction
Study on the Sliding Friction of Endothelial Cells Cultured on Hydrogel and the Role of Glycocalyx on Friction Reduction
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DOI：
10.1002/adem.201080021
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发表时间：
2010-11-01
影响因子：
3.6
通讯作者：
Ando, Joji
中科院分区：
文献类型：
--
作者：
Chen, Yong Mei;Kurokawa, Takayuki;Ando, Joji
我们研究了内皮细胞在PNaSS凝胶上培养的单层细胞的滑动摩擦，旨在阐明内皮细胞表面的糖基化在减摩中的作用。研究了三组HUVEC单层：原代培养的HUVEC单层；经转化生长因子-β(1)处理的HUVEC单层(1)，其糖基化程度增加148%；经肝素酶I处理的HUVEC单层，其糖基化程度降低57%。当在平坦的玻璃表面上滑动时，HUVEC单层的摩擦应力按以下顺序递减：肝素酶I处理>；培养>；转化生长因子-β(1)处理的样品。结果提示，糖萼可能具有降低内皮细胞单层摩擦力的作用。
We investigate the sliding friction of HUVEC monolayers cultured on PNaSS gel, intending to elucidate the role of glycocalyx on the surface of ECs in friction reduction. Three sets of HUVEC monolayers are investigated: an as-cultured HUVEC monolayer; a HUVEC monolayer treated with TGF-beta(1), which increases the glycocalyx by 148%; and a HUVEC monolayer treated with heparinase I, which reduces the glycocalyx by 57%. When being slid on a flat, glass surface, the frictional stress of the HUVEC monolayer decreases in the order: heparinase-I-treated> as-cultured> TGF-beta(1)-treated samples. The results suggest that glycocalyx may play a role in reducing the friction of endothelial cell monolayer.